PXD002002 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Giardia duodenalis Encystation Strain Variation |
Description | Giardia duodenalis is a protozoan parasite responsible for gastroenteritis in vertebrates, including humans. The prevalence of G. duodenalis is partly owed to its direct and simple life cycle, as well as the formation of the environmentally resistant and infective cysts. Several proteomic and transcriptomic studies have previously analysed global changes during the encystation process using the well-characterised laboratory isolate and genome strain, WBC6. To expand current comparative analyses, this study presents the first quantitative global study of encystation using pathogenically relevant and alternative assemblage A strains: the human-derived BRIS/82/HEPU/106 and avian-derived BRIS/95/HEPU/2041. We have utilised tandem MS/MS with a label-free quantitative approach to compare cysts and trophozoite life stages between strains for variation, as well as confirm universal encystation markers of Assemblage A. |
HostingRepository | PRIDE |
AnnounceDate | 2015-06-10 |
AnnouncementXML | Submission_2015-06-10_03:45:41.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Samantha Emery |
SpeciesList | scientific name: Giardia intestinalis assemblage A; NCBI TaxID: 941442; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-03-31 06:07:27 | ID requested | |
⏵ 1 | 2015-06-10 03:45:42 | announced | |
Publication List
Emery SJ, Pascovi D, Lacey E, Haynes PA, The generation gap: Proteome changes and strain variation during encystation in Giardia duodenalis. Mol Biochem Parasitol, 201(1):47-56(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Giardia, LC-MS/MS, Spectral Counting, SpC, NSAF, Label-free, parasitology, protozoans |
Contact List
Paul A Haynes |
contact affiliation | Chemistry and Biomolecular Sciences, Macquarie University, North Ryde |
contact email | paul.haynes@mq.edu.au |
lab head | |
Samantha Emery |
contact affiliation | Chemistry and Biomolecular Sciences |
contact email | samantha.emery@mq.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002002
- Label: PRIDE project
- Name: Giardia duodenalis Encystation Strain Variation