PXD002002

PXD002002 is an original dataset announced via ProteomeXchange.

Title	Giardia duodenalis Encystation Strain Variation
Description	Giardia duodenalis is a protozoan parasite responsible for gastroenteritis in vertebrates, including humans. The prevalence of G. duodenalis is partly owed to its direct and simple life cycle, as well as the formation of the environmentally resistant and infective cysts. Several proteomic and transcriptomic studies have previously analysed global changes during the encystation process using the well-characterised laboratory isolate and genome strain, WBC6. To expand current comparative analyses, this study presents the first quantitative global study of encystation using pathogenically relevant and alternative assemblage A strains: the human-derived BRIS/82/HEPU/106 and avian-derived BRIS/95/HEPU/2041. We have utilised tandem MS/MS with a label-free quantitative approach to compare cysts and trophozoite life stages between strains for variation, as well as confirm universal encystation markers of Assemblage A.
HostingRepository	PRIDE
AnnounceDate	2015-06-10
AnnouncementXML	Submission_2015-06-10_03:45:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samantha Emery
SpeciesList	scientific name: Giardia intestinalis assemblage A; NCBI TaxID: 941442;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-31 06:07:27	ID requested
⏵ 1	2015-06-10 03:45:42	announced

Emery SJ, Pascovi D, Lacey E, Haynes PA, The generation gap: Proteome changes and strain variation during encystation in Giardia duodenalis. Mol Biochem Parasitol, 201(1):47-56(2015) [pubmed]

curator keyword: Biological

submitter keyword: Giardia, LC-MS/MS, Spectral Counting, SpC, NSAF, Label-free, parasitology, protozoans

Paul A Haynes
contact affiliation	Chemistry and Biomolecular Sciences, Macquarie University, North Ryde
contact email	paul.haynes@mq.edu.au
lab head
Samantha Emery
contact affiliation	Chemistry and Biomolecular Sciences
contact email	samantha.emery@mq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002002
     1. Label: PRIDE project
     2. Name: Giardia duodenalis Encystation Strain Variation