PXD001999 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Triple SILAC glycoproteomic analysis of NSCLC cell lines |
Description | Protein glycosylation plays a fundamental role in a multitude of biological processes, and the associated aberrant expression of glycoproteins in cancer has made them attractive targets as biomarkers and therapeutic targets. In this study, we examined differentially expressed glycoproteins in cell lines derived from three different states of lung tumorigenesis: an immortalized bronchial epithelial cell (HBE) line, a non-small cell lung cancer (NSCLC) cell line harboring an activation KRAS mutation and a NSCLC cell line harboring an EGFR activation deletion. Mutations in KRAS and EGFR are two common, distinct, non-overlapping genomic alterations in NSCLC. Using a Triple SILAC proteomic quantification strategy paired with hydrazide chemistry N-linked glycopeptide enrichment, we identified 118 quantifiable glycopeptides in the 3 cell lines derived from 82 glycoproteins. Proteomic profiling revealed that 27 (24%) of the glycopeptides overexpressed in both of the NSCLC cell lines with 6 of the glycopeptides overexpressed only in the EGFR mutant cells and 19 of the glycopeptides overexpressed only in the KRAS mutant cells. |
HostingRepository | PRIDE |
AnnounceDate | 2015-12-10 |
AnnouncementXML | Submission_2015-12-10_04:18:53.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | David Clark |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | deamidated residue; 6x(13)C: 4x(15)N labeled L-arginine; iodoacetamide derivatized residue; 6x(13)C labeled residue; 6x(13)C: 2x(15)N labeled L-lysine; (4: 4 |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-03-31 01:10:36 | ID requested | |
⏵ 1 | 2015-12-10 04:18:54 | announced | |
2 | 2016-03-21 06:52:32 | announced | Updated publication reference for PubMed record(s): 26722374. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Lung cancer, Glycoproteomics, SILAC, SPEG |
Contact List
Li Mao |
contact affiliation | Univesity of Maryland Baltimore, School of Dentistry, Department of Oncology and Diagnostic Sciences |
contact email | LMao@umaryland.edu |
lab head | |
David Clark |
contact affiliation | UMB School of Dentistry |
contact email | david.clark@umaryland.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001999
- Label: PRIDE project
- Name: Triple SILAC glycoproteomic analysis of NSCLC cell lines