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PXD001999

PXD001999 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTriple SILAC glycoproteomic analysis of NSCLC cell lines
DescriptionProtein glycosylation plays a fundamental role in a multitude of biological processes, and the associated aberrant expression of glycoproteins in cancer has made them attractive targets as biomarkers and therapeutic targets. In this study, we examined differentially expressed glycoproteins in cell lines derived from three different states of lung tumorigenesis: an immortalized bronchial epithelial cell (HBE) line, a non-small cell lung cancer (NSCLC) cell line harboring an activation KRAS mutation and a NSCLC cell line harboring an EGFR activation deletion. Mutations in KRAS and EGFR are two common, distinct, non-overlapping genomic alterations in NSCLC. Using a Triple SILAC proteomic quantification strategy paired with hydrazide chemistry N-linked glycopeptide enrichment, we identified 118 quantifiable glycopeptides in the 3 cell lines derived from 82 glycoproteins. Proteomic profiling revealed that 27 (24%) of the glycopeptides overexpressed in both of the NSCLC cell lines with 6 of the glycopeptides overexpressed only in the EGFR mutant cells and 19 of the glycopeptides overexpressed only in the KRAS mutant cells.
HostingRepositoryPRIDE
AnnounceDate2015-12-10
AnnouncementXMLSubmission_2016-03-21_06:52:31.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDavid Clark
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListdeamidated residue; 6x(13)C: 4x(15)N labeled L-arginine; iodoacetamide derivatized residue; 6x(13)C labeled residue; 6x(13)C: 2x(15)N labeled L-lysine; (4: 4
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-03-31 01:10:36ID requested
12015-12-10 04:18:54announced
22016-03-21 06:52:32announcedUpdated publication reference for PubMed record(s): 26722374.
Publication List
Clark DJ, Mei Y, Sun S, Zhang H, Yang AJ, Mao L, Glycoproteomic Approach Identifies KRAS as a Positive Regulator of CREG1 in Non-small Cell Lung Cancer Cells. Theranostics, 6(1):65-77(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Lung cancer, Glycoproteomics, SILAC, SPEG
Contact List
Li Mao
contact affiliationUnivesity of Maryland Baltimore, School of Dentistry, Department of Oncology and Diagnostic Sciences
contact emailLMao@umaryland.edu
lab head
David Clark
contact affiliationUMB School of Dentistry
contact emaildavid.clark@umaryland.edu
dataset submitter
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Dataset FTP location
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