PXD001908 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The fission yeast MTREC complex targets CUTs and unspliced mRNAs to the nuclear exosome |
Description | Cryptic unstable transcripts (CUTs) are rapidly degraded by the nuclear exosome. However, the mechanism by which they are recognized and targeted to the exosome is not fully understood. Here, we report that the MTREC complex, which has recently been shown to promote degradation of meiotic mRNAs and regulatory ncRNAs, is also the major nuclear exosome targeting complex for CUTs and unspliced pre-mRNAs in Schizosaccharomyces pombe. MTREC complex specifically binds to CUTs, meiotic mRNAs and unspliced pre-mRNA transcripts and targets these RNAs for degradation by the nuclear exosome, while the TRAMP complex has only a minor role in this process. The MTREC complex physically interacts with the nuclear exosome and with various RNA-binding and -processing complexes, coupling RNA processing to the RNA degradation machinery. Our study reveals the central role of the evolutionarily conserved MTREC complex in RNA quality control, and in the recognition and elimination of CUTs. |
HostingRepository | PRIDE |
AnnounceDate | 2015-04-22 |
AnnouncementXML | Submission_2015-04-22_08:13:23.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tamas Fischer |
SpeciesList | scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-03-12 02:49:55 | ID requested | |
⏵ 1 | 2015-04-22 08:13:23 | announced | |
2 | 2015-06-01 03:46:04 | announced | Updated publication reference for PubMed record(s): 25989903. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: ncRNA, CUTs, RNA decay, RNA surveillance, TRAMP, MTREC, splicing |
Contact List
Dr. Tamas Fischer |
contact affiliation | AG Fischer BZH University Heidelberg INF 328 69120 Heidelberg Germany |
contact email | tamas.fischer@bzh.uni-heidelberg.de |
lab head | |
Tamas Fischer |
contact affiliation | BZH Heidelberg University |
contact email | tamas.fischer@bzh.uni-heidelberg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/04/PXD001908 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001908
- Label: PRIDE project
- Name: The fission yeast MTREC complex targets CUTs and unspliced mRNAs to the nuclear exosome