PXD001908

PXD001908 is an original dataset announced via ProteomeXchange.

Title	The fission yeast MTREC complex targets CUTs and unspliced mRNAs to the nuclear exosome
Description	Cryptic unstable transcripts (CUTs) are rapidly degraded by the nuclear exosome. However, the mechanism by which they are recognized and targeted to the exosome is not fully understood. Here, we report that the MTREC complex, which has recently been shown to promote degradation of meiotic mRNAs and regulatory ncRNAs, is also the major nuclear exosome targeting complex for CUTs and unspliced pre-mRNAs in Schizosaccharomyces pombe. MTREC complex specifically binds to CUTs, meiotic mRNAs and unspliced pre-mRNA transcripts and targets these RNAs for degradation by the nuclear exosome, while the TRAMP complex has only a minor role in this process. The MTREC complex physically interacts with the nuclear exosome and with various RNA-binding and -processing complexes, coupling RNA processing to the RNA degradation machinery. Our study reveals the central role of the evolutionarily conserved MTREC complex in RNA quality control, and in the recognition and elimination of CUTs.
HostingRepository	PRIDE
AnnounceDate	2015-04-22
AnnouncementXML	Submission_2015-06-01_03:46:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamas Fischer
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-03-12 02:49:55	ID requested
1	2015-04-22 08:13:23	announced
⏵ 2	2015-06-01 03:46:04	announced	Updated publication reference for PubMed record(s): 25989903.

Zhou Y, Zhu J, Schermann G, Ohle C, Bendrin K, Sugioka-Sugiyama R, Sugiyama T, Fischer T, The fission yeast MTREC complex targets CUTs and unspliced pre-mRNAs to the nuclear exosome. Nat Commun, 6():7050(2015) [pubmed]

curator keyword: Biological

submitter keyword: ncRNA, CUTs, RNA decay, RNA surveillance, TRAMP, MTREC, splicing

Dr. Tamas Fischer
contact affiliation	AG Fischer BZH University Heidelberg INF 328 69120 Heidelberg Germany
contact email	tamas.fischer@bzh.uni-heidelberg.de
lab head
Tamas Fischer
contact affiliation	BZH Heidelberg University
contact email	tamas.fischer@bzh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/04/PXD001908

PRIDE project URI

• PRIDE
  1. PXD001908
     1. Label: PRIDE project
     2. Name: The fission yeast MTREC complex targets CUTs and unspliced mRNAs to the nuclear exosome