PXD001882 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Arabidopsis endomembrane enrichments: Affinity enrichment ofYFP-RABD2a/ARA5 |
Description | Our aim was to dissect subcellular trafficking routes by enriching for partially overlapping subpopulations of endosomal proteomes associated with endomembrane markers. We selected RABD2a/ARA5, RABF2b/ARA7, RABF1/ARA6, and RABG3f as markers for combinations of the Golgi, trans-Golgi Network (TGN), Early Endosomes (EE), secretory vesicles, Late Endosomes (LE), Multivesicular Bodies (MVB) compartments and tonoplast. As comparisons we used Golgi transport 1 (GOT1), which localises to the Golgi, Clathrin Light Chain 2 (CLC2) labelling Clathrin-coated vesicles and pits and the Vesicle Associated Membrane Protein 711 (VAMP711) present at the tonoplast. We developed an easy-to-use method by refining published protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker proteins in Arabidopsis thaliana seedlings. |
HostingRepository | PRIDE |
AnnounceDate | 2015-04-22 |
AnnouncementXML | Submission_2015-04-22_07:27:01.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD001882 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alex Jones |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-03-06 05:33:27 | ID requested | |
⏵ 1 | 2015-04-22 07:27:02 | announced | |
2 | 2015-04-27 03:16:40 | announced | Updated publication reference for PubMed record(s): 25900983. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Arabidopsis, affnity purification, clathin, small GTPases, |
Contact List
Alexandra M. E. Jones |
contact affiliation | School of Life Sciences, University of Warwick |
contact email | alex.jones@warwick.ac.uk |
lab head | |
Alex Jones |
contact affiliation | University of Warwick |
contact email | alex.jones@warwick.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001882
- Label: PRIDE project
- Name: Arabidopsis endomembrane enrichments: Affinity enrichment ofYFP-RABD2a/ARA5