PXD001798-1

PXD001798 is an original dataset announced via ProteomeXchange.

Title	System-Wide Identification of Wild-Type SUMO-2 Conjugation Sites
Description	SUMOylation is a reversible post-translational modification regulating all nuclear processes. Identification of SUMOylation sites by mass spectrometry has been hampered by bulky tryptic fragments, which thus far necessitated the use of mutated SUMO. Here, we present a dataset generated through a SUMO-specific protease-based methodology which circumvents this problem, dubbed Protease-Reliant Identification of SUMO Modification (PRISM). PRISM allows for detection of SUMOylated proteins as well as identification of specific sites of SUMOylation while using wild-type SUMO. The method is generic and could be widely applied to study lysine post-translational modifications. PRISM was employed in combination with high-resolution mass spectrometry to identify SUMOylation sites from HeLa cells under standard growth conditions and in response to heat shock. 751 wild-type SUMOylation sites on endogenous proteins were identified, including 200 dynamic SUMO sites in response to heat shock. Thus, we have developed the first method capable of quantitatively studying wild-type mammalian SUMO at the site-specific and system-wide level.
HostingRepository	PRIDE
AnnounceDate	2015-06-15
AnnouncementXML	Submission_2015-06-15_07:32:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivo Hendriks
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	sumoylated lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-09 05:11:39	ID requested
⏵ 1	2015-06-15 07:32:11	announced
2	2015-06-16 12:16:24	announced	Updated publication reference for PubMed record(s): 26073453.

Dataset with its publication pending

curator keyword: Biological, Technical

submitter keyword: SUMO-2, Wild-type, Endogenous, Site, System-wide, SUMO

Alfred C.O. Vertegaal
contact affiliation	Department of Molecular Cell Biology, Leiden University Medical Center, The Netherlands
contact email	a.c.o.vertegaal@lumc.nl
lab head
Ivo Hendriks
contact affiliation	Leiden University Medical Center
contact email	ivo.a.hendriks@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/06/PXD001798

PRIDE project URI

• PRIDE
  1. PXD001798
     1. Label: PRIDE project
     2. Name: System-Wide Identification of Wild-Type SUMO-2 Conjugation Sites