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PXD001798

PXD001798 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSystem-Wide Identification of Wild-Type SUMO-2 Conjugation Sites
DescriptionSUMOylation is a reversible post-translational modification regulating all nuclear processes. Identification of SUMOylation sites by mass spectrometry has been hampered by bulky tryptic fragments, which thus far necessitated the use of mutated SUMO. Here, we present a dataset generated through a SUMO-specific protease-based methodology which circumvents this problem, dubbed Protease-Reliant Identification of SUMO Modification (PRISM). PRISM allows for detection of SUMOylated proteins as well as identification of specific sites of SUMOylation while using wild-type SUMO. The method is generic and could be widely applied to study lysine post-translational modifications. PRISM was employed in combination with high-resolution mass spectrometry to identify SUMOylation sites from HeLa cells under standard growth conditions and in response to heat shock. 751 wild-type SUMOylation sites on endogenous proteins were identified, including 200 dynamic SUMO sites in response to heat shock. Thus, we have developed the first method capable of quantitatively studying wild-type mammalian SUMO at the site-specific and system-wide level.
HostingRepositoryPRIDE
AnnounceDate2015-06-15
AnnouncementXMLSubmission_2015-06-16_12:16:22.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIvo Hendriks
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListsumoylated lysine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-02-09 05:11:39ID requested
12015-06-15 07:32:11announced
22015-06-16 12:16:24announcedUpdated publication reference for PubMed record(s): 26073453.
Publication List
Hendriks IA, D'Souza RC, Chang JG, Mann M, Vertegaal AC, System-wide identification of wild-type SUMO-2 conjugation sites. Nat Commun, 6():7289(2015) [pubmed]
Keyword List
curator keyword: Biological, Technical
submitter keyword: SUMO-2, Wild-type, Endogenous, Site, System-wide, SUMO
Contact List
Alfred C.O. Vertegaal
contact affiliationDepartment of Molecular Cell Biology, Leiden University Medical Center, The Netherlands
contact emaila.c.o.vertegaal@lumc.nl
lab head
Ivo Hendriks
contact affiliationLeiden University Medical Center
contact emailivo.a.hendriks@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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