PXD001786 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics of Alzheimer's Disease mouse models - control dataset |
Description | Alzheimer’s disease (AD) is a neurodegenerative disease displaying plaques formed by the neurotoxic amyloid β-peptide (Aβ), and intracellular neurofibrillary tangles consisting of protein tau. However, how these pathologies relates to the massive neuronal death that occurs in AD brains remain elusive. Neprilysin is the major A degrading enzyme and a lack thereof increases A levels in the brain twofold. To identify altered protein expression levels induced by increased Aβ levels, we performed a proteomic analysis of the brain of the AD mouse model APPsw and compared it to that of APPsw mice lacking neprilysin. To this end we established an LC-MS/MS method to analyze brain homogenate, using an 18O-labeled internal standard to accurately quantify the protein levels. To distinguish between alterations in protein levels directly caused by increased A levels and those induced by neprilysin deficiency independently of A, the brain proteome of neprilysin deficient APPsw mice was compared to that of neprilysin deficient mice. By this approach we identified approximately 600 proteins and could quantify the levels of 300 of these. Pathway analysis showed that many of the proteins with altered expression were involved in neurological disorders, and that tau, presenilin and APP were key regulators in the identified networks. The data have been deposited to the ProteomeXchange Consortium with identifier PXD000968. Interestingly, the levels of several proteins, including some not previously reported to be associated with AD, were associated with increased A levels. |
HostingRepository | PRIDE |
AnnounceDate | 2015-07-29 |
AnnouncementXML | Submission_2015-07-29_08:04:00.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD001786 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Per Nilsson |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | Oxidation; Label:18O(2); Label:18O(1) |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-02-05 03:07:59 | ID requested | |
⏵ 1 | 2015-07-29 08:04:02 | announced | |
Publication List
Nilsson P, Loganathan K, Sekiguchi M, Winblad B, Iwata N, Saido TC, Tjernberg LO, Loss of neprilysin alters protein expression in the brain of Alzheimer's disease model mice. Proteomics, 15(19):3349-55(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: mouse brain |
Contact List
Per Nilsson |
contact affiliation | Lab for Proteolytic Neuroscience, RIKEN Brain Science Institute |
contact email | per-nilsson@brain.riken.jp |
lab head | |
Per Nilsson |
contact affiliation | RIKEN Brain Science Institute |
contact email | per-nilsson@brain.riken.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001786
- Label: PRIDE project
- Name: Proteomics of Alzheimer's Disease mouse models - control dataset