PXD001786

PXD001786 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Alzheimer's Disease mouse models - control dataset
Description	Alzheimer’s disease (AD) is a neurodegenerative disease displaying plaques formed by the neurotoxic amyloid β-peptide (Aβ), and intracellular neurofibrillary tangles consisting of protein tau. However, how these pathologies relates to the massive neuronal death that occurs in AD brains remain elusive. Neprilysin is the major A degrading enzyme and a lack thereof increases A levels in the brain twofold. To identify altered protein expression levels induced by increased Aβ levels, we performed a proteomic analysis of the brain of the AD mouse model APPsw and compared it to that of APPsw mice lacking neprilysin. To this end we established an LC-MS/MS method to analyze brain homogenate, using an 18O-labeled internal standard to accurately quantify the protein levels. To distinguish between alterations in protein levels directly caused by increased A levels and those induced by neprilysin deficiency independently of A, the brain proteome of neprilysin deficient APPsw mice was compared to that of neprilysin deficient mice. By this approach we identified approximately 600 proteins and could quantify the levels of 300 of these. Pathway analysis showed that many of the proteins with altered expression were involved in neurological disorders, and that tau, presenilin and APP were key regulators in the identified networks. The data have been deposited to the ProteomeXchange Consortium with identifier PXD000968. Interestingly, the levels of several proteins, including some not previously reported to be associated with AD, were associated with increased A levels.
HostingRepository	PRIDE
AnnounceDate	2015-07-29
AnnouncementXML	Submission_2015-07-29_08:04:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001786
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Per Nilsson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Label:18O(2); Label:18O(1)
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-02-05 03:07:59	ID requested
⏵ 1	2015-07-29 08:04:02	announced

Nilsson P, Loganathan K, Sekiguchi M, Winblad B, Iwata N, Saido TC, Tjernberg LO, Loss of neprilysin alters protein expression in the brain of Alzheimer's disease model mice. Proteomics, 15(19):3349-55(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: mouse brain

Per Nilsson
contact affiliation	Lab for Proteolytic Neuroscience, RIKEN Brain Science Institute
contact email	per-nilsson@brain.riken.jp
lab head
Per Nilsson
contact affiliation	RIKEN Brain Science Institute
contact email	per-nilsson@brain.riken.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001786
     1. Label: PRIDE project
     2. Name: Proteomics of Alzheimer's Disease mouse models - control dataset