PXD001479

PXD001479 is an original dataset announced via ProteomeXchange.

Title	The gut microbiota affects the mucus barrier
Description	Two C57BL/6 mice colonies maintained in two rooms in the same specific pathogen free (SPF) facility were found to have different gut microbiota and a mucus phenotype specific for each colony. The thickness and growth of the colon mucus was similar in the two colonies, but one colony had mucus not penetrable to bacteria or bacterial-sized beads, similar to what occurs in free-living wild mice. On the other hand, the other colony had an inner mucus layer that was penetrable to bacteria and beads. These different properties of the mucus in the two rooms were dependent on the microbiota, as the phenotypes were transmissible by transfer of ceacal microbiota to germ-free mice. Mice with an impenetrable mucus layer had increased amounts of Erysipelotrichi, while mice with a penetrable mucus layer had higher levels of Proteobacteria and TM7 bacteria in the distal colon mucus. Thus bacteria affect mucus barrier properties in ways that can have implications for health and disease.
HostingRepository	PRIDE
AnnounceDate	2015-02-09
AnnouncementXML	Submission_2015-02-09_11:03:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana M. Rodriguez-Pineiro
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-12 02:29:20	ID requested
⏵ 1	2015-02-09 11:03:06	announced

Jakobsson HE, Rodr, í, guez-Pi, ñ, eiro AM, Sch, ü, tte A, Ermund A, Boysen P, Bemark M, Sommer F, B, ä, ckhed F, Hansson GC, Johansson ME, The composition of the gut microbiota shapes the colon mucus barrier. EMBO Rep, 16(2):164-77(2015) [pubmed]

curator keyword: Biological

submitter keyword: Mucus, ileum, colon, microbiota

Gunnar C. Hansson
contact affiliation	Department of Medical Biochemistry and Cell Biology University of Gothenburg Sweden
contact email	gunnar.hansson@medkem.gu.se
lab head
Ana M. Rodriguez-Pineiro
contact affiliation	Biomedicinska inst
contact email	ana.rodriguez@medkem.gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/02/PXD001479

PRIDE project URI

• PRIDE
  1. PXD001479
     1. Label: PRIDE project
     2. Name: The gut microbiota affects the mucus barrier