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PXD001464 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMultiphasic chip performance characterization using human thyroid cancer cell line SW1736 and human plasma
DescriptionDevelopment of an online peptide fractionation system comprising a multiphasic LC chip that integrates reversed phase and strong cation exchange chromatography upstream of the MS based on the multi-dimensional protein identification technology (mudPIT). Chips were assessed in terms of chromatographic as well as protein and peptide identification reproducibility using five-step fractionation of 0, 2, 50, 500 and 1500 mM ammonium acetate. Further, a comparison of multiphase LC chip protein and peptide identification versus conventional reversed phase LC-MSMS was performed.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristoph Krisp
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; dehydrated residue; acetylated residue; iodoacetamide derivatized residue; phosphorylated residue; deaminated residue; formylated residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-11-05 02:41:21ID requested
12015-04-15 07:42:02announced
Publication List
Krisp C, Yang H, van Soest R, Molloy MP, Online Peptide fractionation using a multiphasic microfluidic liquid chromatography chip improves reproducibility and detection limits for quantitation in discovery and targeted proteomics. Mol Cell Proteomics, 14(6):1708-19(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Chip LC-MSMS, Multiphasic LC chip separation, thyroid cancer cell line, human plasma
Contact List
Assoc Prof Mark P Molloy
contact affiliationMacquarie University Australian Proteome Analysis Facility (APAF) Sydney, Australia
contact emailmmolloy@proteome.org.au
lab head
Christoph Krisp
contact affiliationAustralian Proteome Analysis Facility (APAF)
contact emailckrisp@proteome.org.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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