PXD001464

PXD001464 is an original dataset announced via ProteomeXchange.

Title	Multiphasic chip performance characterization using human thyroid cancer cell line SW1736 and human plasma
Description	Development of an online peptide fractionation system comprising a multiphasic LC chip that integrates reversed phase and strong cation exchange chromatography upstream of the MS based on the multi-dimensional protein identification technology (mudPIT). Chips were assessed in terms of chromatographic as well as protein and peptide identification reproducibility using five-step fractionation of 0, 2, 50, 500 and 1500 mM ammonium acetate. Further, a comparison of multiphase LC chip protein and peptide identification versus conventional reversed phase LC-MSMS was performed.
HostingRepository	PRIDE
AnnounceDate	2015-04-15
AnnouncementXML	Submission_2015-04-15_07:42:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001464
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christoph Krisp
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; dehydrated residue; acetylated residue; iodoacetamide derivatized residue; phosphorylated residue; deaminated residue; formylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-05 02:41:21	ID requested
⏵ 1	2015-04-15 07:42:02	announced

Krisp C, Yang H, van Soest R, Molloy MP, Online Peptide fractionation using a multiphasic microfluidic liquid chromatography chip improves reproducibility and detection limits for quantitation in discovery and targeted proteomics. Mol Cell Proteomics, 14(6):1708-19(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Chip LC-MSMS, Multiphasic LC chip separation, thyroid cancer cell line, human plasma

Assoc Prof Mark P Molloy
contact affiliation	Macquarie University Australian Proteome Analysis Facility (APAF) Sydney, Australia
contact email	mmolloy@proteome.org.au
lab head
Christoph Krisp
contact affiliation	Australian Proteome Analysis Facility (APAF)
contact email	ckrisp@proteome.org.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001464
     1. Label: PRIDE project
     2. Name: Multiphasic chip performance characterization using human thyroid cancer cell line SW1736 and human plasma