PXD001391 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Metastasis-related Intracellular Proteins in Human Breast Cancer Cells |
Description | We have used a unique metastasis model system in combination with quantitative, comparative proteomics to identify novel markers associated with the ability of cancer cells to colonize and form metastasis, and subsequently analyzed the clinical relevance of these proteins using breast cancer biopsies from patients with known clinical outcome within a 10-year follow-up. The model system consists of two isogenic human breast cancer cell lines that are equally tumorigenic in mice, but one gives rise to metastasis while the other disseminates single cells that remain dormant in distant organs. Using stable isotopic labeling by amino acids in cell culture and subcellular fractionation, the nuclear, cytosol and mitochondria proteomes were analyzed by LC-MS/MS, identifying seven proteins that exhibited altered expression between the cell lines, including L-lactate dehydrogenase A, NADH-cytochrome b5 reductase 3 (CYB5R3), niemann-pick c1 protein, and nucleolar RNA helicase 2. |
HostingRepository | PRIDE |
AnnounceDate | 2015-09-14 |
AnnouncementXML | Submission_2015-09-14_08:13:02.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rikke Raaen Lund |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue; 6x(13)C labeled residue |
Instrument | Waters instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-10-09 05:51:54 | ID requested | |
⏵ 1 | 2015-09-14 08:13:03 | announced | |
Publication List
Lund RR, Leth-Larsen R, Caterino TD, Terp MG, Nissen J, L, æ, nkholm AV, Jensen ON, Ditzel HJ, NADH-Cytochrome b5 Reductase 3 Promotes Colonization and Metastasis Formation and Is a Prognostic Marker of Disease-Free and Overall Survival in Estrogen Receptor-Negative Breast Cancer. Mol Cell Proteomics, 14(11):2988-99(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Human, Breast Cancer, Metastasis, ESI-QTOF, SILAC |
Contact List
Henrik Jørn Ditzel |
contact affiliation | Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark |
contact email | hditzel@health.sdu.dk |
lab head | |
Rikke Raaen Lund |
contact affiliation | University of Southern Denmark |
contact email | rlund@health.sdu.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001391
- Label: PRIDE project
- Name: Metastasis-related Intracellular Proteins in Human Breast Cancer Cells