PXD001391

PXD001391 is an original dataset announced via ProteomeXchange.

Title	Metastasis-related Intracellular Proteins in Human Breast Cancer Cells
Description	We have used a unique metastasis model system in combination with quantitative, comparative proteomics to identify novel markers associated with the ability of cancer cells to colonize and form metastasis, and subsequently analyzed the clinical relevance of these proteins using breast cancer biopsies from patients with known clinical outcome within a 10-year follow-up. The model system consists of two isogenic human breast cancer cell lines that are equally tumorigenic in mice, but one gives rise to metastasis while the other disseminates single cells that remain dormant in distant organs. Using stable isotopic labeling by amino acids in cell culture and subcellular fractionation, the nuclear, cytosol and mitochondria proteomes were analyzed by LC-MS/MS, identifying seven proteins that exhibited altered expression between the cell lines, including L-lactate dehydrogenase A, NADH-cytochrome b5 reductase 3 (CYB5R3), niemann-pick c1 protein, and nucleolar RNA helicase 2.
HostingRepository	PRIDE
AnnounceDate	2015-09-14
AnnouncementXML	Submission_2015-09-14_08:13:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rikke Raaen Lund
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; 6x(13)C labeled residue
Instrument	Waters instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-09 05:51:54	ID requested
⏵ 1	2015-09-14 08:13:03	announced

Lund RR, Leth-Larsen R, Caterino TD, Terp MG, Nissen J, L, æ, nkholm AV, Jensen ON, Ditzel HJ, NADH-Cytochrome b5 Reductase 3 Promotes Colonization and Metastasis Formation and Is a Prognostic Marker of Disease-Free and Overall Survival in Estrogen Receptor-Negative Breast Cancer. Mol Cell Proteomics, 14(11):2988-99(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, Breast Cancer, Metastasis, ESI-QTOF, SILAC

Henrik Jørn Ditzel
contact affiliation	Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark
contact email	hditzel@health.sdu.dk
lab head
Rikke Raaen Lund
contact affiliation	University of Southern Denmark
contact email	rlund@health.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/09/PXD001391

PRIDE project URI

• PRIDE
  1. PXD001391
     1. Label: PRIDE project
     2. Name: Metastasis-related Intracellular Proteins in Human Breast Cancer Cells