PXD001385-2

PXD001385 is an original dataset announced via ProteomeXchange.

Title	Intensity-based label-free proteomics using a quadrupole orbitrap mass spectrometer
Description	We present a unique data set for benchmarking label free quantitative proteomics using a quadrupole orbitrap mass spectrometer. Soluble Escherichia coli digest was spiked into a HeLa digest in four different concentrations, simulating protein expression differences. The data set, which is available online, provides a unique opportunity to test the proteomic platform (instrumentation and software) in its ability to perform intensity based label free quantification. We show that the presented combination of informatics and instrumentation produces high precision and quantification accuracy.
HostingRepository	PRIDE
AnnounceDate	2015-03-27
AnnouncementXML	Submission_2015-03-27_01:30:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yishai Levin
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-08 03:05:54	ID requested
1	2015-03-25 07:34:03	announced
⏵ 2	2015-03-27 01:30:53	announced	Updated project metadata.

Shalit T, Elinger D, Savidor A, Gabashvili A, Levin Y, MS1-based label-free proteomics using a quadrupole orbitrap mass spectrometer. J Proteome Res, 14(4):1979-86(2015) [pubmed]

curator keyword: Technical

submitter keyword: Proteomics, Q Exactive, Quadrupole Orbitrap, label-free

Yishai Levin
contact affiliation	de Botton Institute for Protein Profiling, The Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science
contact email	yishai.levin@weizmann.ac.il
lab head
Yishai Levin
contact affiliation	Weizmann Institute of Science, Israel
contact email	yishai.levin@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/03/PXD001385

PRIDE project URI

• PRIDE
  1. PXD001385
     1. Label: PRIDE project
     2. Name: Intensity-based label-free proteomics using a quadrupole orbitrap mass spectrometer