PXD001257 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Shotgun ecotoxicoproteomics of Daphnia pulex: biochemical effects of the anticancer drug tamoxifen |
Description | Among pollutants released into the environment by human activities, residues of pharmaceutical agents have been identified worldwide, and are an increasing matter of concern because of their potential impact on ecosystems. Because micro-crustaceans, such as daphnids, are sensitive to chemicals and are key organisms in the food chain, they are particularly convenient for studying the effects of these drugs in the aquatic environment. The aim of this study was to analyse differences of protein expression resulting from acute (2 days) and middle-term (7 days) exposure of Daphnia pulex daphnids to the anticancer drug tamoxifen, with the hope to unveil links between the effects of xenobiotics at the organism level and changes at the molecular level. Using a liquid chromatography - mass spectrometry shotgun approach, about 4000 proteins could be identified with a minimum of one unique peptide, providing the largest proteomics dataset of D. pulex published up to now. Considering both time points and tested concentrations, 189 proteins showed a significant fold change, most of the regulated proteins being observed at the highest concentration tested and for the longer exposure time. About one third of significant proteins were positively regulated, whereas two thirds showed decreased levels after tamoxifen treatment. The identity of regulated proteins suggested a decrease in translation, an increase in protein degradation and changes in carbohydrate and lipid metabolism as the major effects of the drug. Besides these impacted processes, which reflect a general stress response of the organism, some other regulated proteins play a role in Daphnia reproduction. These latter results are in accordance with our previous observations of the impact of tamoxifen on D. pulex reproduction, and illustrate the potential of ecotoxicoproteomics to unravel links between xenobiotic effects at the biochemical and at the organismal level. |
HostingRepository | PRIDE |
AnnounceDate | 2014-10-31 |
AnnouncementXML | Submission_2014-10-31_05:27:18.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Patrice Waridel |
SpeciesList | scientific name: Daphnia pulex (Water flea); NCBI TaxID: 6669; |
ModificationList | deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-08-22 05:28:12 | ID requested | |
⏵ 1 | 2014-10-31 05:27:19 | announced | |
Publication List
Borgatta M, Hernandez C, Decosterd LA, Ch, รจ, vre N, Waridel P, Shotgun ecotoxicoproteomics of Daphnia pulex: biochemical effects of the anticancer drug tamoxifen. J Proteome Res, 14(1):279-91(2015) [pubmed] |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: Daphnia pulex, LC-MS/MS, tamoxifen, ecotoxiproteomics |
Contact List
Manfredo Quadroni |
contact affiliation | Protein Analysis Facility, Center for Integrative Genomics, University of Lausanne |
contact email | manfredo.quadroni@unil.ch |
lab head | |
Patrice Waridel |
contact affiliation | University of Lausanne |
contact email | patrice.waridel@unil.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001257
- Label: PRIDE project
- Name: Shotgun ecotoxicoproteomics of Daphnia pulex: biochemical effects of the anticancer drug tamoxifen