PXD001251-2

PXD001251 is an original dataset announced via ProteomeXchange.

Title	Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions
Description	To identify long-lived proteins that are retained in yeast mother cells, we combined a method developed in our lab with a pulse-chase protocol using total proteome analysis. Cells were initially grown in medium with stable heavy-isotope amino acids to label proteins. The cells were then switched into media containing light-isotope amino acids and cultured for an additional ~18 cell divisions with the MEP engaged. Finally, we determined whether any of the original heavy-labeled proteins were still present in the aged mother cells
HostingRepository	PRIDE
AnnounceDate	2014-09-01
AnnouncementXML	Submission_2014-09-25_03:37:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nathaniel Thayer
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	6x(13)C labeled residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-20 01:43:44	ID requested
1	2014-09-01 07:11:52	announced
⏵ 2	2014-09-25 03:37:23	announced	Updated publication reference for PubMed record(s): 25228775.

Thayer NH, Leverich CK, Fitzgibbon MP, Nelson ZW, Henderson KA, Gafken PR, Hsu JJ, Gottschling DE, Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions. Proc Natl Acad Sci U S A, 111(39):14019-26(2014) [pubmed]

curator keyword: Biological

submitter keyword: saccharomyces cerevisiae, SILAC, replicative aging

Daniel E. Gottschling
contact affiliation	Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA USA
contact email	dgottsch@fhcrc.org
lab head
Nathaniel Thayer
contact affiliation	FHCRC
contact email	nthayer@fhcrc.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/09/PXD001251

PRIDE project URI

• PRIDE
  1. PXD001251
     1. Label: PRIDE project
     2. Name: Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions