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PXD001251

PXD001251 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of long-lived proteins retained in cells undergoing repeated asymmetric divisions
DescriptionTo identify long-lived proteins that are retained in yeast mother cells, we combined a method developed in our lab with a pulse-chase protocol using total proteome analysis. Cells were initially grown in medium with stable heavy-isotope amino acids to label proteins. The cells were then switched into media containing light-isotope amino acids and cultured for an additional ~18 cell divisions with the MEP engaged. Finally, we determined whether any of the original heavy-labeled proteins were still present in the aged mother cells
HostingRepositoryPRIDE
AnnounceDate2014-09-01
AnnouncementXMLSubmission_2014-09-25_03:37:22.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNathaniel Thayer
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList6x(13)C labeled residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-08-20 01:43:44ID requested
12014-09-01 07:11:52announced
22014-09-25 03:37:23announcedUpdated publication reference for PubMed record(s): 25228775.
Publication List
Thayer NH, Leverich CK, Fitzgibbon MP, Nelson ZW, Henderson KA, Gafken PR, Hsu JJ, Gottschling DE, Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions. Proc Natl Acad Sci U S A, 111(39):14019-26(2014) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: saccharomyces cerevisiae, SILAC, replicative aging
Contact List
Daniel E. Gottschling
contact affiliationBasic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA USA
contact emaildgottsch@fhcrc.org
lab head
Nathaniel Thayer
contact affiliationFHCRC
contact emailnthayer@fhcrc.org
dataset submitter
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PRIDE project URI
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