PXD001251 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions |
Description | To identify long-lived proteins that are retained in yeast mother cells, we combined a method developed in our lab with a pulse-chase protocol using total proteome analysis. Cells were initially grown in medium with stable heavy-isotope amino acids to label proteins. The cells were then switched into media containing light-isotope amino acids and cultured for an additional ~18 cell divisions with the MEP engaged. Finally, we determined whether any of the original heavy-labeled proteins were still present in the aged mother cells |
HostingRepository | PRIDE |
AnnounceDate | 2014-09-01 |
AnnouncementXML | Submission_2014-09-25_03:37:22.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nathaniel Thayer |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | 6x(13)C labeled residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-08-20 01:43:44 | ID requested | |
1 | 2014-09-01 07:11:52 | announced | |
⏵ 2 | 2014-09-25 03:37:23 | announced | Updated publication reference for PubMed record(s): 25228775. |
Publication List
Thayer NH, Leverich CK, Fitzgibbon MP, Nelson ZW, Henderson KA, Gafken PR, Hsu JJ, Gottschling DE, Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions. Proc Natl Acad Sci U S A, 111(39):14019-26(2014) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: saccharomyces cerevisiae, SILAC, replicative aging |
Contact List
Daniel E. Gottschling |
contact affiliation | Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA USA |
contact email | dgottsch@fhcrc.org |
lab head | |
Nathaniel Thayer |
contact affiliation | FHCRC |
contact email | nthayer@fhcrc.org |
dataset submitter | |
Full Dataset Link List
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001251
- Label: PRIDE project
- Name: Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions