PXD001251

PXD001251 is an original dataset announced via ProteomeXchange.

Title	Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions
Description	To identify long-lived proteins that are retained in yeast mother cells, we combined a method developed in our lab with a pulse-chase protocol using total proteome analysis. Cells were initially grown in medium with stable heavy-isotope amino acids to label proteins. The cells were then switched into media containing light-isotope amino acids and cultured for an additional ~18 cell divisions with the MEP engaged. Finally, we determined whether any of the original heavy-labeled proteins were still present in the aged mother cells
HostingRepository	PRIDE
AnnounceDate	2014-09-01
AnnouncementXML	Submission_2014-09-25_03:37:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nathaniel Thayer
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	6x(13)C labeled residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-20 01:43:44	ID requested
1	2014-09-01 07:11:52	announced
⏵ 2	2014-09-25 03:37:23	announced	Updated publication reference for PubMed record(s): 25228775.

Thayer NH, Leverich CK, Fitzgibbon MP, Nelson ZW, Henderson KA, Gafken PR, Hsu JJ, Gottschling DE, Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions. Proc Natl Acad Sci U S A, 111(39):14019-26(2014) [pubmed]

curator keyword: Biological

submitter keyword: saccharomyces cerevisiae, SILAC, replicative aging

Daniel E. Gottschling
contact affiliation	Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA USA
contact email	dgottsch@fhcrc.org
lab head
Nathaniel Thayer
contact affiliation	FHCRC
contact email	nthayer@fhcrc.org
dataset submitter

Dataset FTP location

PRIDE project URI

• PRIDE
  1. PXD001251
     1. Label: PRIDE project
     2. Name: Identification of long-lived proteins retained in cells undergoing repeated asymmetric divisions