PXD001226-2

PXD001226 is an original dataset announced via ProteomeXchange.

Title	Protein deacetylation affects acetate metabolism, motility and acid stress response in Escherichia coli
Description	Although protein acetylation is widely observed, it has been associated with few specific regulatory functions making it poorly understood. To interrogate its functionality, we analyzed the acetylome in Escherichia coli knockout mutants of cobB, the only known sirtuin-like deacetylase, and patZ, the best-known protein acetyltransferase. For four growth conditions, more than 2,000 unique acetylated peptides, belonging to 809 proteins, were identified and differentially quantified. Nearly 65% of these proteins are related to metabolism. The global activity of CobB contributes to the deacetylation of a large number of substrates and has a major impact on physiology. Apart from the regulation of acetyl-CoA synthetase, we found that CobB-controlled acetylation of isocitrate lyase contributes to the fine-tuning of the glyoxylate shunt. Acetylation of the transcription factor RcsB prevents DNA binding, activating flagella biosynthesis and motility, and increases acid stress susceptibility. Surprisingly, deletion of patZ increased acetylation in acetate cultures, which suggests that it regulates the levels of acetylating agents. The results presented offer new insights into functional roles of protein acetylation in metabolic fitness and global cell regulation.
HostingRepository	PRIDE
AnnounceDate	2014-09-30
AnnouncementXML	Submission_2015-03-16_07:41:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harm Post
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap Velos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-14 03:38:27	ID requested
1	2014-09-30 03:09:36	announced
⏵ 2	2015-03-16 07:41:34	announced	Updated publication reference for PubMed record(s): 25518064.

Casta, ñ, o-Cerezo S, Bernal V, Post H, Fuhrer T, Cappadona S, S, á, nchez-D, í, az NC, Sauer U, Heck AJ, Altelaar AF, C, á, novas M, Protein acetylation affects acetate metabolism, motility and acid stress response in Escherichia coli. Mol Syst Biol, 10(11):762(2014) [pubmed]

curator keyword: Biological

submitter keyword: protein acetylation, sirtuins, motility, acetate metabolism, glyoxylate shunt

Manuel Cánovas
contact affiliation	Departamento de Bioquímica y Biología Molecular B e Inmunología. Facultad de Química. Universidad de Murcia. Campus de Excelencia Mare Nostrum
contact email	mcanovas@um.es
lab head
Harm Post
contact affiliation	Biomolecular Mass Spectrometry and Proteomics Group
contact email	h.post@uu.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001226
     1. Label: PRIDE project
     2. Name: Protein deacetylation affects acetate metabolism, motility and acid stress response in Escherichia coli