PXD001199 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Systematic identification of hypothetical bacteriophage proteins targeting key protein complexes of Pseudomonas aeruginosa |
Description | Addressing the functionality of predicted genes remains an enormous challenge in the post-genomic era. A prime example of genes lacking functional assignments are the poorly conserved, early expressed genes of lytic bacteriophages, whose products are involved in the subversion of the host metabolism. In this study, we focused on the composition of important macromolecular complexes of Pseudomonas aeruginosa involved in transcription, DNA replication, fatty acid biosynthesis, RNA regulation, energy metabolism and cell division, during infection with members of seven distinct clades of lytic phages. Using affinity purifications of these host protein complexes coupled to mass spectrometric analyses, 37 host complex-associated phage proteins could be identified. Importantly, eight of these show an inhibitory effect on bacterial growth upon episomal expression, suggesting that these phage proteins are potentially involved in hijacking the host complexes. Using complementary protein-protein interaction assays, we further mapped the inhibitory interaction of gp12 of phage 14-1 to the α subunit of the RNA polymerase. Together, our data demonstrate the powerful use of interactomics to unravel the biological role of hypothetical phage proteins, which constitute an enormous untapped source of novel antibacterial proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2014-09-09 |
AnnouncementXML | Submission_2014-09-09_06:21:42.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jean-Paul Noben |
SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ; LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-08-05 02:14:46 | ID requested | |
⏵ 1 | 2014-09-09 06:21:44 | announced | |
2 | 2014-10-23 05:01:12 | announced | Updated project metadata. |
Publication List
Van den Bossche A, Ceyssens PJ, De Smet J, Hendrix H, Bellon H, Leimer N, Wagemans J, Delattre AS, Cenens W, Aertsen A, Landuyt B, Minakhin L, Severinov K, Noben JP, Lavigne R, Systematic identification of hypothetical bacteriophage proteins targeting key protein complexes of Pseudomonas aeruginosa. J Proteome Res, 13(10):4446-56(2014) [pubmed] |
Keyword List
submitter keyword: Bacteriophages, P. aeruginosa, Interactomics, Affinity purifications, Functional annotation |
Contact List
Prof Rob Lavigne |
contact affiliation | Division of Gene Technology, KU Leuven, B-3001 Leuven, Belgium |
contact email | rob.lavigne@biw.kuleuven.be |
lab head | |
Jean-Paul Noben |
contact affiliation | FBI |
contact email | jeanpaul.noben@uhasselt.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001199
- Label: PRIDE project
- Name: Systematic identification of hypothetical bacteriophage proteins targeting key protein complexes of Pseudomonas aeruginosa