PXD001199

PXD001199 is an original dataset announced via ProteomeXchange.

Title	Systematic identification of hypothetical bacteriophage proteins targeting key protein complexes of Pseudomonas aeruginosa
Description	Addressing the functionality of predicted genes remains an enormous challenge in the post-genomic era. A prime example of genes lacking functional assignments are the poorly conserved, early expressed genes of lytic bacteriophages, whose products are involved in the subversion of the host metabolism. In this study, we focused on the composition of important macromolecular complexes of Pseudomonas aeruginosa involved in transcription, DNA replication, fatty acid biosynthesis, RNA regulation, energy metabolism and cell division, during infection with members of seven distinct clades of lytic phages. Using affinity purifications of these host protein complexes coupled to mass spectrometric analyses, 37 host complex-associated phage proteins could be identified. Importantly, eight of these show an inhibitory effect on bacterial growth upon episomal expression, suggesting that these phage proteins are potentially involved in hijacking the host complexes. Using complementary protein-protein interaction assays, we further mapped the inhibitory interaction of gp12 of phage 14-1 to the α subunit of the RNA polymerase. Together, our data demonstrate the powerful use of interactomics to unravel the biological role of hypothetical phage proteins, which constitute an enormous untapped source of novel antibacterial proteins.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_05:01:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jean-Paul Noben
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-05 02:14:46	ID requested
1	2014-09-09 06:21:44	announced
⏵ 2	2014-10-23 05:01:12	announced	Updated project metadata.

Van den Bossche A, Ceyssens PJ, De Smet J, Hendrix H, Bellon H, Leimer N, Wagemans J, Delattre AS, Cenens W, Aertsen A, Landuyt B, Minakhin L, Severinov K, Noben JP, Lavigne R, Systematic identification of hypothetical bacteriophage proteins targeting key protein complexes of Pseudomonas aeruginosa. J Proteome Res, 13(10):4446-56(2014) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Bacteriophages, P. aeruginosa, Interactomics, Affinity purifications, Functional annotation

Prof Rob Lavigne
contact affiliation	Division of Gene Technology, KU Leuven, B-3001 Leuven, Belgium
contact email	rob.lavigne@biw.kuleuven.be
lab head
Jean-Paul Noben
contact affiliation	FBI
contact email	jeanpaul.noben@uhasselt.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/09/PXD001199

PRIDE project URI

• PRIDE
  1. PXD001199
     1. Label: PRIDE project
     2. Name: Systematic identification of hypothetical bacteriophage proteins targeting key protein complexes of Pseudomonas aeruginosa