PXD001120 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Adenovirus particles SILAC analysis of different batches and mutant virus |
Description | We used stable isotope labelling of amino acids in cell culture coupled with high throughput quantitative mass spectrometry to analyse the protein composition of highly purified wild type adenoviruses, mutant adenoviruses lacking an internal protein component (protein V) and recombinant adenoviruses of the type commonly used in gene therapy including one virus which had been used in a clinical trail. We found that the viral protein abundance and composition was consistent across all types of virus examined except for the virus lacking protein V which also had reduced amounts of another viral core protein, protein VII. In all the samples analysed we found no evidence of consistent packaging or contamination with cellular proteins. We believe this technique makes a powerful method to analyse the protein composition of this important gene therapy vector and genetically engineered or synthetic virus like particles. |
HostingRepository | PRIDE |
AnnounceDate | 2014-10-23 |
AnnouncementXML | Submission_2014-10-23_05:00:07.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | David Matthews |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 6x(13)C: 4x(15)N labeled L-arginine; 6x(13)C labeled residue; 6x(13)C: 2x(15)N labeled L-lysine |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-07-09 07:46:39 | ID requested | |
1 | 2014-08-08 07:50:16 | announced | |
⏵ 2 | 2014-10-23 05:00:08 | announced | Updated project metadata. |
Publication List
Alqahtani A, Heesom K, Bramson JL, Curiel D, Ugai H, Matthews DA, Analysis of purified wild type and mutant adenovirus particles by SILAC based quantitative proteomics. J Gen Virol, 95(Pt 11):2504-2511(2014) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: human adenovirus purified particles |
Contact List
David Matthews |
contact affiliation | Cellular and Molecular Medicine University of Bristol Bristol BS8 1TD |
contact email | d.a.matthews@bristol.ac.uk |
lab head | |
David Matthews |
contact affiliation | University of Bristol |
contact email | d.a.matthews@bristol.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD001120 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001120
- Label: PRIDE project
- Name: Adenovirus particles SILAC analysis of different batches and mutant virus