PXD001120

PXD001120 is an original dataset announced via ProteomeXchange.

Title	Adenovirus particles SILAC analysis of different batches and mutant virus
Description	We used stable isotope labelling of amino acids in cell culture coupled with high throughput quantitative mass spectrometry to analyse the protein composition of highly purified wild type adenoviruses, mutant adenoviruses lacking an internal protein component (protein V) and recombinant adenoviruses of the type commonly used in gene therapy including one virus which had been used in a clinical trail. We found that the viral protein abundance and composition was consistent across all types of virus examined except for the virus lacking protein V which also had reduced amounts of another viral core protein, protein VII. In all the samples analysed we found no evidence of consistent packaging or contamination with cellular proteins. We believe this technique makes a powerful method to analyse the protein composition of this important gene therapy vector and genetically engineered or synthetic virus like particles.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_05:00:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Matthews
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C: 4x(15)N labeled L-arginine; 6x(13)C labeled residue; 6x(13)C: 2x(15)N labeled L-lysine
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-09 07:46:39	ID requested
1	2014-08-08 07:50:16	announced
⏵ 2	2014-10-23 05:00:08	announced	Updated project metadata.

Alqahtani A, Heesom K, Bramson JL, Curiel D, Ugai H, Matthews DA, Analysis of purified wild type and mutant adenovirus particles by SILAC based quantitative proteomics. J Gen Virol, 95(Pt 11):2504-2511(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: human adenovirus purified particles

David Matthews
contact affiliation	Cellular and Molecular Medicine University of Bristol Bristol BS8 1TD
contact email	d.a.matthews@bristol.ac.uk
lab head
David Matthews
contact affiliation	University of Bristol
contact email	d.a.matthews@bristol.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD001120

PRIDE project URI

• PRIDE
  1. PXD001120
     1. Label: PRIDE project
     2. Name: Adenovirus particles SILAC analysis of different batches and mutant virus