PXD001078 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Middle infrared radiation on human metastatic breast cancer MDA-MB-231 cells |
Description | Breast cancer is one of the cancer-related leading causes of death worldwide. Treatment of breast cancer is complex and challenging especially when metastasis is developed. In this study, we established a new concept of using infrared radiation as an alternative approach to breast cancer treatment. We used middle infrared (MIR) in the wavelength range of 3 to 5 μm to irradiate breast cancer cells. MIR significantly inhibited cell proliferation in several breast cancer cells, but did not affect the growth of normal breast epithelium cells. We performed iTRAQ-coupled LC-MS/MS system to investigate the MIR-triggered molecular mechanisms in breast cancer cells. A total of 1,749 proteins were quantified and 167 proteins were considered to be regulated by MIR. Applying the functional enrichment analysis on the proteomics results, we confirmed that MIR caused G2/M cell cycle arrest, remodeled microtubule network to an astral pole arrangement, altered actin filament formation and focal adhesion molecule localization, and reduced cell migration activity and invasion ability. Together, our results uncover the collaborative effects of MIR regulated physiological responses in concentrated networks, demonstrating the potential implementation of infrared radiation in breast cancer therapy. |
HostingRepository | PRIDE |
AnnounceDate | 2015-06-23 |
AnnouncementXML | Submission_2015-06-23_05:02:19.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hsin-Yi Chang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; iTRAQ4plex-116 reporter+balance reagent acylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-06-23 01:23:42 | ID requested | |
⏵ 1 | 2015-06-23 05:02:20 | announced | |
Publication List
Chang HY, Li MH, Huang TC, Hsu CL, Tsai SR, Lee SC, Huang HC, Juan HF, Quantitative proteomics reveals middle infrared radiation-interfered networks in breast cancer cells. J Proteome Res, 14(2):1250-62(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Human, MDA-MB-231, LTQ Orbitraq |
Contact List
Hsueh-Fen Juan |
contact affiliation | Department of Life Science, National Taiwan University |
contact email | yukijuan@ntu.edu.tw |
lab head | |
Hsin-Yi Chang |
contact affiliation | National Taiwan University |
contact email | hsinyi.chang@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001078
- Label: PRIDE project
- Name: Middle infrared radiation on human metastatic breast cancer MDA-MB-231 cells