PXD001078

PXD001078 is an original dataset announced via ProteomeXchange.

Title	Middle infrared radiation on human metastatic breast cancer MDA-MB-231 cells
Description	Breast cancer is one of the cancer-related leading causes of death worldwide. Treatment of breast cancer is complex and challenging especially when metastasis is developed. In this study, we established a new concept of using infrared radiation as an alternative approach to breast cancer treatment. We used middle infrared (MIR) in the wavelength range of 3 to 5 μm to irradiate breast cancer cells. MIR significantly inhibited cell proliferation in several breast cancer cells, but did not affect the growth of normal breast epithelium cells. We performed iTRAQ-coupled LC-MS/MS system to investigate the MIR-triggered molecular mechanisms in breast cancer cells. A total of 1,749 proteins were quantified and 167 proteins were considered to be regulated by MIR. Applying the functional enrichment analysis on the proteomics results, we confirmed that MIR caused G2/M cell cycle arrest, remodeled microtubule network to an astral pole arrangement, altered actin filament formation and focal adhesion molecule localization, and reduced cell migration activity and invasion ability. Together, our results uncover the collaborative effects of MIR regulated physiological responses in concentrated networks, demonstrating the potential implementation of infrared radiation in breast cancer therapy.
HostingRepository	PRIDE
AnnounceDate	2015-06-23
AnnouncementXML	Submission_2015-06-23_05:02:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hsin-Yi Chang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-23 01:23:42	ID requested
⏵ 1	2015-06-23 05:02:20	announced

Chang HY, Li MH, Huang TC, Hsu CL, Tsai SR, Lee SC, Huang HC, Juan HF, Quantitative proteomics reveals middle infrared radiation-interfered networks in breast cancer cells. J Proteome Res, 14(2):1250-62(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, MDA-MB-231, LTQ Orbitraq

Hsueh-Fen Juan
contact affiliation	Department of Life Science, National Taiwan University
contact email	yukijuan@ntu.edu.tw
lab head
Hsin-Yi Chang
contact affiliation	National Taiwan University
contact email	hsinyi.chang@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/06/PXD001078

PRIDE project URI

• PRIDE
  1. PXD001078
     1. Label: PRIDE project
     2. Name: Middle infrared radiation on human metastatic breast cancer MDA-MB-231 cells