PXD001047 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Additional precursor purification in isobaric mass tagging experiments by traveling wave ion mobility separation (TWIMS). |
Description | We investigated if additional peptide purification by traveling wave ion mobility separation (TWIMS) can reduce precursor contamination using a mixture of S. cerevisiae and HeLa proteomes. In accordance with previous reports on FAIMS-Orbitrap instruments, we find that TWIMS provides a remarkable improvement (on average 2.85 times) in signal to noise ratio for sequence ions. We also report, that TWIMS reduces reporter ions contamination by around one third (to 14 - 15% contamination) and even further (to 6-9%) when combined with narrowed quadrupole isolation window. |
HostingRepository | PRIDE |
AnnounceDate | 2014-06-10 |
AnnouncementXML | Submission_2014-07-16_10:02:28.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pavel Shliaha |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-06-09 02:51:49 | ID requested | |
1 | 2014-06-10 03:31:06 | announced | |
⏵ 2 | 2014-07-16 10:02:29 | announced | Updated publication reference for PubMed record(s): 24854137. |
3 | 2014-07-24 05:19:38 | announced | Updated project metadata. |
Publication List
Shliaha PV, Jukes-Jones R, Christoforou A, Fox J, Hughes C, Langridge J, Cain K, Lilley KS, Additional precursor purification in isobaric mass tagging experiments by traveling wave ion mobility separation (TWIMS). J Proteome Res, 13(7):3360-9(2014) [pubmed] |
Keyword List
submitter keyword: ion mobility separation, IMS, travelling wave ion mobility separation, TWIMS, isobaric mass tag, tandem mass tag, TMT |
Contact List
Kathryn Susan Lilley |
contact affiliation | Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, UK |
contact email | ksl23@cam.ac.uk |
lab head | |
Pavel Shliaha |
contact affiliation | Cambridge Center For Proteomics |
contact email | pvs22@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001047
- Label: PRIDE project
- Name: Additional precursor purification in isobaric mass tagging experiments by traveling wave ion mobility separation (TWIMS).