PXD001047

PXD001047 is an original dataset announced via ProteomeXchange.

Title	Additional precursor purification in isobaric mass tagging experiments by traveling wave ion mobility separation (TWIMS).
Description	We investigated if additional peptide purification by traveling wave ion mobility separation (TWIMS) can reduce precursor contamination using a mixture of S. cerevisiae and HeLa proteomes. In accordance with previous reports on FAIMS-Orbitrap instruments, we find that TWIMS provides a remarkable improvement (on average 2.85 times) in signal to noise ratio for sequence ions. We also report, that TWIMS reduces reporter ions contamination by around one third (to 14 - 15% contamination) and even further (to 6-9%) when combined with narrowed quadrupole isolation window.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_05:19:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Shliaha
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-09 02:51:49	ID requested
1	2014-06-10 03:31:06	announced
2	2014-07-16 10:02:29	announced	Updated publication reference for PubMed record(s): 24854137.
⏵ 3	2014-07-24 05:19:38	announced	Updated project metadata.

Shliaha PV, Jukes-Jones R, Christoforou A, Fox J, Hughes C, Langridge J, Cain K, Lilley KS, Additional precursor purification in isobaric mass tagging experiments by traveling wave ion mobility separation (TWIMS). J Proteome Res, 13(7):3360-9(2014) [pubmed]

curator keyword: Technical

submitter keyword: ion mobility separation, IMS, travelling wave ion mobility separation, TWIMS, isobaric mass tag, tandem mass tag, TMT

Kathryn Susan Lilley
contact affiliation	Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, UK
contact email	ksl23@cam.ac.uk
lab head
Pavel Shliaha
contact affiliation	Cambridge Center For Proteomics
contact email	pvs22@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/06/PXD001047

PRIDE project URI

• PRIDE
  1. PXD001047
     1. Label: PRIDE project
     2. Name: Additional precursor purification in isobaric mass tagging experiments by traveling wave ion mobility separation (TWIMS).