PXD001019-2

PXD001019 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomic Analysis Reveals Posttranslational Responses to Aneuploidy in Yeast
Description	Aneuploidy causes severe developmental defects and is a near universal feature of tumor cells. Despite its profound effects, the cellular processes affected by aneuploidy are not well characterized. Here, we examined the consequences of aneuploidy on the proteome of aneuploid budding yeast strains. We show that although protein levels largely scale with gene copy number, subunits of multi-protein complexes are notable exceptions. Posttranslational mechanisms attenuate their expression when their encoding genes are in excess. Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis. Indeed aneuploid cells harbor increased levels of reactive oxygen species (ROS). Interestingly, increased protein turnover attenuates ROS levels and this novel aneuploidy-associated signature and improves the fitness of most aneuploid strains. Our results show that aneuploidy causes alterations in metabolism and redox homeostasis. Cells respond to these alterations through both transcriptional and posttranscriptional mechanisms.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_03:55:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Noah Dephoure
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-29 01:47:05	ID requested
1	2014-08-14 03:45:28	announced
⏵ 2	2014-10-23 03:55:57	announced	Updated project metadata.

Dephoure N, Hwang S, O'Sullivan C, Dodgson SE, Gygi SP, Amon A, Torres EM, Quantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast. Elife, 3():e03023(2014) [pubmed]

curator keyword: Biological

submitter keyword: saccharomyces cerevisiae, aneuploidy, SILAC, TMT

Noah Dephoure
contact affiliation	Weill Cornell Medical College Department of Biochemistry
contact email	nod2007@med.cornell.edu
lab head
Noah Dephoure
contact affiliation	Weill Cornell Medical College Department of Biochemistry
contact email	nod2007@med.cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD001019

PRIDE project URI

• PRIDE
  1. PXD001019
     1. Label: PRIDE project
     2. Name: Quantitative Proteomic Analysis Reveals Posttranslational Responses to Aneuploidy in Yeast