PXD000968 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics of Alzheimer's Disease mouse models |
Description | Alzheimer’s disease (AD) is a neurodegenerative disease displaying plaques formed by the neurotoxic amyloid β-peptide (Aβ) and intracellular neurofibrillary tangles consisting of protein tau. However, how these pathologies relates to the massive neuronal death that occurs in AD brains remain elusive. To identify proteins, the levels of which are affected by increased Aβ levels, we performed a proteomic analysis of the AD mouse model APPsw and compared it to that of APPsw mice lacking the major Aβ degrading enzyme neprilysin. This was achieved by establishing an LC-MS/MS method to analyze brain homogenate, using an 18O-labeled internal standard to accurately quantify the protein levels. By this approach we identified approximately 600 proteins and could quantify the levels of 300 of these. Interestingly, several proteins, including some not previously reported to be associated with AD, were identified. The relevance of these proteins for AD warrants further research. |
HostingRepository | PRIDE |
AnnounceDate | 2015-07-29 |
AnnouncementXML | Submission_2015-07-29_07:49:13.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000968 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Per Nilsson |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | Oxidation; Label:18O(2); Label:18O(1) |
Instrument | LTQ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-05-12 05:34:09 | ID requested | |
⏵ 1 | 2015-07-29 07:49:15 | announced | |
Publication List
Nilsson P, Loganathan K, Sekiguchi M, Winblad B, Iwata N, Saido TC, Tjernberg LO, Loss of neprilysin alters protein expression in the brain of Alzheimer's disease model mice. Proteomics, 15(19):3349-55(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Alzheimer Disease, mosue models, brain, LC-MS |
Contact List
Lars Tjernberg |
contact affiliation | Karolinska Institutet, Center for Alzheimer Research, Dept of Neurobiology, Care Sciences and Society, 141 57, Huddinge, Sweden |
contact email | lars.tjernberg@ki.se |
lab head | |
Per Nilsson |
contact affiliation | RIKEN Brain Science Institute |
contact email | per-nilsson@brain.riken.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000968
- Label: PRIDE project
- Name: Proteomics of Alzheimer's Disease mouse models