PXD000968

PXD000968 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Alzheimer's Disease mouse models
Description	Alzheimer’s disease (AD) is a neurodegenerative disease displaying plaques formed by the neurotoxic amyloid β-peptide (Aβ) and intracellular neurofibrillary tangles consisting of protein tau. However, how these pathologies relates to the massive neuronal death that occurs in AD brains remain elusive. To identify proteins, the levels of which are affected by increased Aβ levels, we performed a proteomic analysis of the AD mouse model APPsw and compared it to that of APPsw mice lacking the major Aβ degrading enzyme neprilysin. This was achieved by establishing an LC-MS/MS method to analyze brain homogenate, using an 18O-labeled internal standard to accurately quantify the protein levels. By this approach we identified approximately 600 proteins and could quantify the levels of 300 of these. Interestingly, several proteins, including some not previously reported to be associated with AD, were identified. The relevance of these proteins for AD warrants further research.
HostingRepository	PRIDE
AnnounceDate	2015-07-29
AnnouncementXML	Submission_2015-07-29_07:49:13.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000968
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Per Nilsson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Label:18O(2); Label:18O(1)
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-12 05:34:09	ID requested
⏵ 1	2015-07-29 07:49:15	announced

Nilsson P, Loganathan K, Sekiguchi M, Winblad B, Iwata N, Saido TC, Tjernberg LO, Loss of neprilysin alters protein expression in the brain of Alzheimer's disease model mice. Proteomics, 15(19):3349-55(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Alzheimer Disease, mosue models, brain, LC-MS

Lars Tjernberg
contact affiliation	Karolinska Institutet, Center for Alzheimer Research, Dept of Neurobiology, Care Sciences and Society, 141 57, Huddinge, Sweden
contact email	lars.tjernberg@ki.se
lab head
Per Nilsson
contact affiliation	RIKEN Brain Science Institute
contact email	per-nilsson@brain.riken.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/07/PXD000968

PRIDE project URI

• PRIDE
  1. PXD000968
     1. Label: PRIDE project
     2. Name: Proteomics of Alzheimer's Disease mouse models