PXD000963 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteins from formalin-fixed paraffin-embedded prostate cancer sections that predict the risk of metastatic disease |
Description | Purpose: Prostate cancer is the most frequently diagnosed cancer in men and the third leading cause of cancer related deaths among men living in developed countries. Biomarkers that predict disease outcome at the time of initial diagnosis would aid management of the disease. Experimental design: Proteins extracted from formalin-fixed paraffin-embeddded sections were identified either using LC-MALDI MS/MS of tryptic digests or after separation by one- or two-dimensional electrophoresis. A list of potential biomarker candidates, based on proposed associations with prostate cancer, was derived from the 320 identified proteins. Results: Four of the candidates: annexin A2, peroxiredoxin-1, prostate-specific antigen and zinc-alpha-2-glycoprotein, were examined by Western blotting of extracts of biopsies from men whom there was 10-year disease outcome data. Multiplexed Western blotting using cocktails of antibodies and fluor-labeled detection reagents showed that annexin A2 provided the best prediction of risk of metastatic disease. Conclusions and clinical relevance: This pilot study indicates that tumour expression of ANXA2 in diagnostic biopsies of a prostate cancer is predictive for the metastatic potential of that cancer. The potential of protein profiling of each cancer is to lead to an overall reduction in mortality from metastatic prostate cancer as well as reduced treatment associated morbidity. |
HostingRepository | PRIDE |
AnnounceDate | 2015-09-14 |
AnnouncementXML | Submission_2016-03-22_03:56:34.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000963 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Danyl Mclauchlan |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | 4800 Proteomics Analyzer |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-05-09 00:52:36 | ID requested | |
1 | 2015-09-14 07:36:07 | announced | |
⏵ 2 | 2016-03-22 03:56:36 | announced | Updated publication reference for PubMed record(s): 26388710. |
3 | 2017-08-03 02:36:20 | announced | Updated project metadata. |
Publication List
Dunne JC, Lamb DS, Delahunt B, Murray J, Bethwaite P, Ferguson P, Nacey JN, Sondhauss S, Jordan TW, Proteins from formalin-fixed paraffin-embedded prostate cancer sections that predict the risk of metastatic disease. Clin Proteomics, 12(1):24(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: FFPE / Prognostic biomarkers / Prostate cancer / Proteomics |
Contact List
Bill Jordan |
contact affiliation | Victoria University of Wellington, New Zealand |
contact email | bill.jordan@vuw.ac.nz |
lab head | |
Danyl Mclauchlan |
contact affiliation | Centre for Biodiscovery |
contact email | danyl.mclauchlan@vuw.ac.nz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000963
- Label: PRIDE project
- Name: Proteins from formalin-fixed paraffin-embedded prostate cancer sections that predict the risk of metastatic disease