PXD000963

PXD000963 is an original dataset announced via ProteomeXchange.

Title	Proteins from formalin-fixed paraffin-embedded prostate cancer sections that predict the risk of metastatic disease
Description	Purpose: Prostate cancer is the most frequently diagnosed cancer in men and the third leading cause of cancer related deaths among men living in developed countries. Biomarkers that predict disease outcome at the time of initial diagnosis would aid management of the disease. Experimental design: Proteins extracted from formalin-fixed paraffin-embeddded sections were identified either using LC-MALDI MS/MS of tryptic digests or after separation by one- or two-dimensional electrophoresis. A list of potential biomarker candidates, based on proposed associations with prostate cancer, was derived from the 320 identified proteins. Results: Four of the candidates: annexin A2, peroxiredoxin-1, prostate-specific antigen and zinc-alpha-2-glycoprotein, were examined by Western blotting of extracts of biopsies from men whom there was 10-year disease outcome data. Multiplexed Western blotting using cocktails of antibodies and fluor-labeled detection reagents showed that annexin A2 provided the best prediction of risk of metastatic disease. Conclusions and clinical relevance: This pilot study indicates that tumour expression of ANXA2 in diagnostic biopsies of a prostate cancer is predictive for the metastatic potential of that cancer. The potential of protein profiling of each cancer is to lead to an overall reduction in mortality from metastatic prostate cancer as well as reduced treatment associated morbidity.
HostingRepository	PRIDE
AnnounceDate	2017-08-03
AnnouncementXML	Submission_2017-08-03_02:36:19.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000963
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Danyl Mclauchlan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-09 00:52:36	ID requested
1	2015-09-14 07:36:07	announced
2	2016-03-22 03:56:36	announced	Updated publication reference for PubMed record(s): 26388710.
⏵ 3	2017-08-03 02:36:20	announced	Updated project metadata.

Dunne JC, Lamb DS, Delahunt B, Murray J, Bethwaite P, Ferguson P, Nacey JN, Sondhauss S, Jordan TW, Proteins from formalin-fixed paraffin-embedded prostate cancer sections that predict the risk of metastatic disease. Clin Proteomics, 12(1):24(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: FFPE / Prognostic biomarkers / Prostate cancer / Proteomics

Bill Jordan
contact affiliation	Victoria University of Wellington, New Zealand
contact email	bill.jordan@vuw.ac.nz
lab head
Danyl Mclauchlan
contact affiliation	Centre for Biodiscovery
contact email	danyl.mclauchlan@vuw.ac.nz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000963
     1. Label: PRIDE project
     2. Name: Proteins from formalin-fixed paraffin-embedded prostate cancer sections that predict the risk of metastatic disease