PXD000892 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Reproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC |
Description | We describe the application of magnetic TiO2 and Ti-IMAC for uniform automated phosphopeptide enrichment. Combining hyper-porous magnetic microspheres with a magnetic particle-handling robot enables rapid (45 minutes), reproducible (r2 = 0.80) and high-fidelity (> 90% purity) phosphopeptide purification in a 96-well format. |
HostingRepository | PRIDE |
AnnounceDate | 2014-09-22 |
AnnouncementXML | Submission_2014-09-22_04:36:20.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christopher Tape |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Velos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-04-10 06:14:44 | ID requested | |
⏵ 1 | 2014-09-22 04:36:21 | announced | |
Publication List
Tape CJ, Worboys JD, Sinclair J, Gourlay R, Vogt J, McMahon KM, Trost M, Lauffenburger DA, Lamont DJ, J, ø, rgensen C, Reproducible automated phosphopeptide enrichment using magnetic TiO2 and Ti-IMAC. Anal Chem, 86(20):10296-302(2014) [pubmed] |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: Phosphoproteomics Automation |
Contact List
Claus Jørgensen |
contact affiliation | Cancer Research UK Manchester Institute |
contact email | claus.jorgensen@cruk.manchester.ac.uk |
lab head | |
Christopher Tape |
contact affiliation | Institute of Cancer Research |
contact email | christopher.tape@icr.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000892
- Label: PRIDE project
- Name: Reproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC