PXD000892

PXD000892 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Reproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC
Description	We describe the application of magnetic TiO2 and Ti-IMAC for uniform automated phosphopeptide enrichment. Combining hyper-porous magnetic microspheres with a magnetic particle-handling robot enables rapid (45 minutes), reproducible (r2 = 0.80) and high-fidelity (> 90% purity) phosphopeptide purification in a 96-well format.
HostingRepository	PRIDE
AnnounceDate	2014-09-22
AnnouncementXML	Submission_2014-09-22_04:36:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Tape
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-10 06:14:44	ID requested
⏵ 1	2014-09-22 04:36:21	announced

Publication List

Tape CJ, Worboys JD, Sinclair J, Gourlay R, Vogt J, McMahon KM, Trost M, Lauffenburger DA, Lamont DJ, J, ø, rgensen C, Reproducible automated phosphopeptide enrichment using magnetic TiO2 and Ti-IMAC. Anal Chem, 86(20):10296-302(2014) [pubmed]

Tape CJ, Worboys JD, Sinclair J, Gourlay R, Vogt J, McMahon KM, Trost M, Lauffenburger DA, Lamont DJ, J, ø, rgensen C, Reproducible automated phosphopeptide enrichment using magnetic TiO2 and Ti-IMAC. Anal Chem, 86(20):10296-302(2014) [pubmed]

Keyword List

curator keyword: Biomedical, Biological
submitter keyword: Phosphoproteomics Automation

curator keyword: Biomedical, Biological

submitter keyword: Phosphoproteomics Automation

Contact List

Claus Jørgensen
contact affiliation	Cancer Research UK Manchester Institute
contact email	claus.jorgensen@cruk.manchester.ac.uk
lab head
Christopher Tape
contact affiliation	Institute of Cancer Research
contact email	christopher.tape@icr.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/09/PXD000892
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/09/PXD000892

PRIDE project URI

Repository Record List

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