PXD000888 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Anoxia in Ralstonia eutropha H16 |
Description | Ralstonia eutropha H16 is a denitrifying microorganism able to use nitrate and nitrite as terminal electron acceptors under oxygen deprivation. To identify proteins showing an altered expression pattern in response to oxygen supply, R. eutropha cells grown aerobically and anaerobically were compared in a comprehensive proteome and transcriptome approach. Nearly 700 proteins involved in several processes including respiration, cell appendages formation, DNA and cofactor biosynthesis were found to be differentially expressed. A combination of 1D-gel-LC and conventional 2D-gel analysis of six consecutive sample points covering the entire denitrification sequence revealed a detailed view on the abundance pattern of the key proteins of denitrification. Denitrification- or anaerobiosis-induced alterations of the respiratory chain included a distinct expression pattern for multiple terminal oxidases. Alterations of the central metabolism were restricted to a few key functions including the isoenzymes for aconitase and isocitrate dehydrogenase, respectively. Although R. eutropha is a strictly respiratory bacterium, the abundance of certain fermentation enzymes was increased. This work represents a comprehensive survey of denitrification on proteomic and transcriptomic level and provides unique insight into how R. eutropha adapts its metabolism to low oxygen conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2014-09-02 |
AnnouncementXML | Submission_2014-09-02_00:30:29.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andreas Otto |
SpeciesList | scientific name: Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha); NCBI TaxID: 381666; |
ModificationList | monohydroxylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-04-10 01:56:16 | ID requested | |
1 | 2014-09-01 08:14:47 | announced | |
⏵ 2 | 2014-09-02 00:30:29 | announced | Updated project metadata. |
3 | 2014-09-25 03:37:55 | announced | Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Ralstonia eutropha H16, spectral counting, membrane proteins, denitrification, anaerobiosis |
Contact List
Andreas Otto |
contact affiliation | Institut fuer Mikrobiologie EMAU Greifswald |
contact email | andreas.otto@uni-greifswald.de |
lab head | |
Andreas Otto |
contact affiliation | Institut f????r Mikrobiologie |
contact email | andreas.otto@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000888
- Label: PRIDE project
- Name: Anoxia in Ralstonia eutropha H16