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PXD000888

PXD000888 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnoxia in Ralstonia eutropha H16
DescriptionRalstonia eutropha H16 is a denitrifying microorganism able to use nitrate and nitrite as terminal electron acceptors under oxygen deprivation. To identify proteins showing an altered expression pattern in response to oxygen supply, R. eutropha cells grown aerobically and anaerobically were compared in a comprehensive proteome and transcriptome approach. Nearly 700 proteins involved in several processes including respiration, cell appendages formation, DNA and cofactor biosynthesis were found to be differentially expressed. A combination of 1D-gel-LC and conventional 2D-gel analysis of six consecutive sample points covering the entire denitrification sequence revealed a detailed view on the abundance pattern of the key proteins of denitrification. Denitrification- or anaerobiosis-induced alterations of the respiratory chain included a distinct expression pattern for multiple terminal oxidases. Alterations of the central metabolism were restricted to a few key functions including the isoenzymes for aconitase and isocitrate dehydrogenase, respectively. Although R. eutropha is a strictly respiratory bacterium, the abundance of certain fermentation enzymes was increased. This work represents a comprehensive survey of denitrification on proteomic and transcriptomic level and provides unique insight into how R. eutropha adapts its metabolism to low oxygen conditions.
HostingRepositoryPRIDE
AnnounceDate2014-09-02
AnnouncementXMLSubmission_2014-09-25_03:37:54.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndreas Otto
SpeciesList scientific name: Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha); NCBI TaxID: 381666;
ModificationListmonohydroxylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-04-10 01:56:16ID requested
12014-09-01 08:14:47announced
22014-09-02 00:30:29announcedUpdated project metadata.
32014-09-25 03:37:55announcedUpdated project metadata.
Publication List
Kohlmann Y, Pohlmann A, Schwartz E, Z, ΓΌ, hlke D, Otto A, Albrecht D, Grimmler C, Ehrenreich A, Voigt B, Becher D, Hecker M, Friedrich B, Cramm R, Coping with anoxia: a comprehensive proteomic and transcriptomic survey of denitrification. J Proteome Res, 13(10):4325-38(2014) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Ralstonia eutropha H16, spectral counting, membrane proteins, denitrification, anaerobiosis
Contact List
Andreas Otto
contact affiliationInstitut fuer Mikrobiologie EMAU Greifswald
contact emailandreas.otto@uni-greifswald.de
lab head
Andreas Otto
contact affiliationInstitut f????r Mikrobiologie
contact emailandreas.otto@uni-greifswald.de
dataset submitter
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Dataset FTP location
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