PXD000875 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The membrane proteome of Medicago truncatula roots displays qualitative and quantitative changes in response to arbuscular mycorrhizal symbiosis |
Description | Arbuscular mycorrhizal (AM) symbiosis that associates roots of most land plants with soilborne fungi (Glomeromycota), is characterized by reciprocal nutritional benefits. Fungal colonization of plant roots induces massive changes in cortical cells where the fungus differentiates an arbuscule, which drives proliferation of the plasma membrane, and the de novo synthesis of the periarbuscular membrane. Despite the recognized importance of membrane proteins in sustaining AM symbiosis, the root microsomal proteome elicited upon mycorrhiza still remains to be explored. In this study, we first examined the qualitative composition of the root membrane proteome of Medicago truncatula after microsome enrichment and subsequent in depth analysis by GeLC-MS/MS. The results obtained highlighted the identification of 1226 root membrane protein candidates whose cellular and functional classifications predispose plastids and protein synthesis as prevalent organelle and function, respectively. Changes at the protein abundance level between the membrane proteomes of mycorrhizal and nonmycorrhizal roots were further monitored by spectral counting, which retrieved a total of 97 proteins that displayed a differential accumulation upon AM symbiosis. Besides the canonical markers of the periarbuscular membrane, new candidates supporting the importance of membrane trafficking events during mycorrhiza establishment/functioning were identified, including flotillin-like proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2014-06-30 |
AnnouncementXML | Submission_2014-06-30_01:37:51.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | cosette abdallah |
SpeciesList | scientific name: Medicago truncatula var. truncatula; NCBI TaxID: 428386; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-04-03 08:00:52 | ID requested | |
⏵ 1 | 2014-06-30 01:37:52 | announced | |
2 | 2014-07-24 05:15:38 | announced | Updated project metadata. |
Publication List
Abdallah C, Valot B, Guillier C, Mounier A, Balliau T, Zivy M, van Tuinen D, Renaut J, Wipf D, Dumas-Gaudot E, Recorbet G, The membrane proteome of Medicago truncatula roots displays qualitative and quantitative changes in response to arbuscular mycorrhizal symbiosis. J Proteomics, 108():354-68(2014) [pubmed] |
Keyword List
submitter keyword: Root membrane proteome, GeLC-MS/MS, Arbuscular mycorrhiza, Spectral counting, Rhizophagus irregularis |
Contact List
Ghislaine Recorbet |
contact affiliation | UMR INRA 1347/AgroSup/Université de Bourgogne, Dijon, France |
contact email | ghislaine.recorbet@dijon.inra.fr |
lab head | |
cosette abdallah |
contact affiliation | INSERM |
contact email | cosette_a@hotmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000875
- Label: PRIDE project
- Name: The membrane proteome of Medicago truncatula roots displays qualitative and quantitative changes in response to arbuscular mycorrhizal symbiosis