PXD000875

PXD000875 is an original dataset announced via ProteomeXchange.

Title	The membrane proteome of Medicago truncatula roots displays qualitative and quantitative changes in response to arbuscular mycorrhizal symbiosis
Description	Arbuscular mycorrhizal (AM) symbiosis that associates roots of most land plants with soilborne fungi (Glomeromycota), is characterized by reciprocal nutritional benefits. Fungal colonization of plant roots induces massive changes in cortical cells where the fungus differentiates an arbuscule, which drives proliferation of the plasma membrane, and the de novo synthesis of the periarbuscular membrane. Despite the recognized importance of membrane proteins in sustaining AM symbiosis, the root microsomal proteome elicited upon mycorrhiza still remains to be explored. In this study, we first examined the qualitative composition of the root membrane proteome of Medicago truncatula after microsome enrichment and subsequent in depth analysis by GeLC-MS/MS. The results obtained highlighted the identification of 1226 root membrane protein candidates whose cellular and functional classifications predispose plastids and protein synthesis as prevalent organelle and function, respectively. Changes at the protein abundance level between the membrane proteomes of mycorrhizal and nonmycorrhizal roots were further monitored by spectral counting, which retrieved a total of 97 proteins that displayed a differential accumulation upon AM symbiosis. Besides the canonical markers of the periarbuscular membrane, new candidates supporting the importance of membrane trafficking events during mycorrhiza establishment/functioning were identified, including flotillin-like proteins.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_05:15:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	cosette abdallah
SpeciesList	scientific name: Medicago truncatula var. truncatula; NCBI TaxID: 428386;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-03 08:00:52	ID requested
1	2014-06-30 01:37:52	announced
⏵ 2	2014-07-24 05:15:38	announced	Updated project metadata.

Abdallah C, Valot B, Guillier C, Mounier A, Balliau T, Zivy M, van Tuinen D, Renaut J, Wipf D, Dumas-Gaudot E, Recorbet G, The membrane proteome of Medicago truncatula roots displays qualitative and quantitative changes in response to arbuscular mycorrhizal symbiosis. J Proteomics, 108():354-68(2014) [pubmed]

curator keyword: Biological

submitter keyword: Root membrane proteome, GeLC-MS/MS, Arbuscular mycorrhiza, Spectral counting, Rhizophagus irregularis

Ghislaine Recorbet
contact affiliation	UMR INRA 1347/AgroSup/Université de Bourgogne, Dijon, France
contact email	ghislaine.recorbet@dijon.inra.fr
lab head
cosette abdallah
contact affiliation	INSERM
contact email	cosette_a@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000875
     1. Label: PRIDE project
     2. Name: The membrane proteome of Medicago truncatula roots displays qualitative and quantitative changes in response to arbuscular mycorrhizal symbiosis