PXD000759 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphopeptide enrichment on synthetic libraries and HeLa cells digest |
Description | 2 phosphopeptide enrichment methods (TiO2 and Ti-IMAC) were evaluated on synthetic libraries and HeLa cells digest. The resulting peptides were compared to the ones without enrichment with regards to the nature of the phosphorylation site (serine, threonine, tyrosine) and the characteristics of the peptide enriched. It was found that the enrichment methods are unbiased for these properties and that the result of the enrichment reflects the original condition of the sample. |
HostingRepository | PRIDE |
AnnounceDate | 2014-10-23 |
AnnouncementXML | Submission_2014-10-23_03:06:01.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lucrece Matheron |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-02-14 03:48:16 | ID requested | |
1 | 2014-08-11 05:17:56 | announced | |
⏵ 2 | 2014-10-23 03:06:02 | announced | Updated project metadata. |
Publication List
Matheron L, van den Toorn H, Heck AJ, Mohammed S, Characterization of biases in phosphopeptide enrichment by Ti(4+)-immobilized metal affinity chromatography and TiO2 using a massive synthetic library and human cell digests. Anal Chem, 86(16):8312-20(2014) [pubmed] |
Keyword List
ProteomeXchange project tag: PRIME-XS Project |
curator keyword: Technical |
submitter keyword: phosphopeptide enrichment, TiO2, Ti-IMAC, synthetic library, HeLa |
Contact List
Albert Heck |
contact affiliation | Utrecht University |
contact email | A.J.R.Heck@uu.nl |
lab head | |
Lucrece Matheron |
contact affiliation | Utrecht University |
contact email | l.i.matheron@uu.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD000759 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000759
- Label: PRIDE project
- Name: Phosphopeptide enrichment on synthetic libraries and HeLa cells digest