PXD000759

PXD000759 is an original dataset announced via ProteomeXchange.

Title	Phosphopeptide enrichment on synthetic libraries and HeLa cells digest
Description	2 phosphopeptide enrichment methods (TiO2 and Ti-IMAC) were evaluated on synthetic libraries and HeLa cells digest. The resulting peptides were compared to the ones without enrichment with regards to the nature of the phosphorylation site (serine, threonine, tyrosine) and the characteristics of the peptide enriched. It was found that the enrichment methods are unbiased for these properties and that the result of the enrichment reflects the original condition of the sample.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_03:06:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lucrece Matheron
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-02-14 03:48:16	ID requested
1	2014-08-11 05:17:56	announced
⏵ 2	2014-10-23 03:06:02	announced	Updated project metadata.

Matheron L, van den Toorn H, Heck AJ, Mohammed S, Characterization of biases in phosphopeptide enrichment by Ti(4+)-immobilized metal affinity chromatography and TiO2 using a massive synthetic library and human cell digests. Anal Chem, 86(16):8312-20(2014) [pubmed]

ProteomeXchange project tag: PRIME-XS Project

curator keyword: Technical

submitter keyword: phosphopeptide enrichment, TiO2, Ti-IMAC, synthetic library, HeLa

Albert Heck
contact affiliation	Utrecht University
contact email	A.J.R.Heck@uu.nl
lab head
Lucrece Matheron
contact affiliation	Utrecht University
contact email	l.i.matheron@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD000759

PRIDE project URI

• PRIDE
  1. PXD000759
     1. Label: PRIDE project
     2. Name: Phosphopeptide enrichment on synthetic libraries and HeLa cells digest