PXD000713 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Plant SILAC: Stable-Isotope Labelling with Amino Acids of Arabidopsis Seedlings for Quantitative Proteomics. |
Description | Stable Isotope Labelling by Amino acids in Cell culture (SILAC) is a powerful technique for comparative quantitative proteomics, which has recently been applied to a number of different eukaryotic organisms. Inefficient incorporation of labelled amino acids in cell cultures of Arabidopsis thaliana has led to very limited use of SILAC in plant systems. We present a method allowing, for the first time, efficient labelling with stable isotope-containing arginine and lysine of whole Arabidopsis seedlings. To illustrate the utility of this method, we have combined the high labelling efficiency (>95%) with quantitative proteomics analyses of seedlings exposed to increased salt concentration. In plants treated for 7 days with 80 mM NaCl, a relatively mild salt stress, 215 proteins were identified whose expression levels changed significantly compared to untreated seedling controls. The 92 up-regulated proteins included proteins involved in abiotic stress responses and photosynthesis, while the 123 down-regulated proteins were enriched in proteins involved in reduction of oxidative stress and other stress responses, respectively. Efficient labelling of whole Arabidopsis seedlings by this modified SILAC method opens new opportunities to exploit the genetic resources of Arabidopsis and analyse the impact of mutations on quantitative protein dynamics in vivo. |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-24 |
AnnouncementXML | Submission_2014-07-24_05:16:43.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Vackar Afzal |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | deamidated residue; oxidized residue; phosphorylated residue; Acetyl; N-acylated residue; 2-pyrrolidone-5-carboxylic acid (Gln) |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-01-24 02:10:46 | ID requested | |
1 | 2014-01-30 01:11:18 | announced | |
⏵ 2 | 2014-07-24 05:16:44 | announced | Updated project metadata. |
Publication List
Lewandowska D, ten Have S, Hodge K, Tillemans V, Lamond AI, Brown JW, Plant SILAC: stable-isotope labelling with amino acids of arabidopsis seedlings for quantitative proteomics. PLoS One, 8(8):e72207(2013) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Silac, Arabidopsis |
Contact List
Prof Angus Lamond |
contact affiliation | College of Life Sciences, University of Dundee |
contact email | a.i.lamond@dundee.ac.uk |
lab head | |
Vackar Afzal |
contact affiliation | School of Life Sciences |
contact email | v.z.afzal@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000713
- Label: PRIDE project
- Name: Plant SILAC: Stable-Isotope Labelling with Amino Acids of Arabidopsis Seedlings for Quantitative Proteomics.