PXD000685-1

PXD000685 is an original dataset announced via ProteomeXchange.

Title	Virotrap: Abducting Protein Complexes from Mammalian Cells
Description	Cell lysis is an inevitable step in classical affinity purification - mass spectrometry (AP-MS) strategies to map intracellular protein interactions. Cell homogenization implies a drastic change of the protein complex environment which strongly impedes comprehensive analysis. Complementary lysis conditions, in situ crosslinking strategies and proximal labeling techniques have been recently developed to address part of this problem. We here demonstrate the use of a viral particle sorting approach as an alternative lysis-free method. By fusing a bait protein to the HIV-1 GAG protein, we show that interaction partners of the bait protein become trapped within virus-like particles (VLPs) that bud from mammalian cells. This so-called Virotrap approach obviates the need for cell homogenization and preserves the protein complexes during purification. Using an efficient VLP enrichment protocol, Virotrap allows the detection of known binary interactions as well as MS-based identification of novel protein partners. In addition, we show the identification of stimulus-dependent interactions and demonstrate trapping of protein partners for small molecules. Virotrap constitutes an elegant complementary approach to the current arsenal of MS-based methods to study protein complexes.
HostingRepository	PRIDE
AnnounceDate	2016-04-27
AnnouncementXML	Submission_2016-04-27_13:34:54.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000685
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sven Eyckerman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue: 42.010565; 2-pyrrolidone-5-carboxylic acid (Gln): -17.026549; monohydroxylated residue: 15.994915
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-01-15 02:20:42	ID requested
⏵ 1	2016-04-27 13:34:55	announced

Dataset with its publication pending

submitter keyword: human VLP protein complex

Sven Eyckerman
contact affiliation	VIB Medical Biotechnology Center, VIB, A. Baertsoenkaai 3 B-9000 Ghent, Belgium
contact email	sven.eyckerman@vib-ugent.be
lab head
Sven Eyckerman
contact affiliation	Medical Protein Research
contact email	sven.eyckerman@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000685
     1. Label: PRIDE project
     2. Name: Virotrap: Abducting Protein Complexes from Mammalian Cells