PXD000605 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The human plasma proteome before and after remote ischemic preconditioning |
Description | Six healthy male adults underwent RIPC, consisting of 3x5 min cycles of upper arm ischemia (inflating a blood pressure cuff to >200 mmHg) alternating with 3x5 min reperfusion (bp cuff deflated). Blood samples were collected at baseline and at 1 and 4 min into each reperfusion period. Plasma was isolated and the six reperfusion samples were pooled to one RIPC sample. Both baseline and RIPC samples were depleted, concentrated, trypsin digested, iTRAQ labeled and analyzed by LC and Orbitrap-MS where each RIPC sample was compared to the same individual's baseline sample. |
HostingRepository | PRIDE |
AnnounceDate | 2014-09-16 |
AnnouncementXML | Submission_2015-06-02_01:55:01.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000605 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Marc Vaudel |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iTRAQ4plex-114 reporter+balance reagent O4'-acylated tyrosine: 144.105918; 2-pyrrolidone-5-carboxylic acid (Glu): -18.010565; acetylated residue: 42.010565; 2-pyrrolidone-5-carboxylic acid (Gln): -17.026549; monohydroxylated residue: 15.994915; iTRAQ4plex-114 reporter+balance reagent N6-acylated lysine: 144.105918; iTRAQ4plex-114 reporter+balance reagent acylated N-terminal: 144.105918; L-cysteine methyl disulfide: 45.987721 |
Instrument | LTQ Orbitrap; instrument model: LTQ-Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-11-27 02:56:48 | ID requested | |
1 | 2014-09-16 06:44:04 | announced | |
⏵ 2 | 2015-06-02 01:55:03 | announced | Updated publication reference for PubMed record(s): 25333471. |
Publication List
Helgeland E, Breivik LE, Vaudel M, Svendsen Ø S, Garberg H, Nordrehaug JE, Berven FS, Jonassen AK, Exploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution. PLoS One, 9(10):e109279(2014) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: human, plasma, remote ischemic preconditioning |
Contact List
Marc Vaudel |
contact affiliation | PROBE Dept of Biomedicine |
contact email | marc.vaudel@biomed.uib.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000605
- Label: PRIDE project
- Name: The human plasma proteome before and after remote ischemic preconditioning