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PXD000605

PXD000605 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe human plasma proteome before and after remote ischemic preconditioning
DescriptionSix healthy male adults underwent RIPC, consisting of 3x5 min cycles of upper arm ischemia (inflating a blood pressure cuff to >200 mmHg) alternating with 3x5 min reperfusion (bp cuff deflated). Blood samples were collected at baseline and at 1 and 4 min into each reperfusion period. Plasma was isolated and the six reperfusion samples were pooled to one RIPC sample. Both baseline and RIPC samples were depleted, concentrated, trypsin digested, iTRAQ labeled and analyzed by LC and Orbitrap-MS where each RIPC sample was compared to the same individual's baseline sample.
HostingRepositoryPRIDE
AnnounceDate2014-09-16
AnnouncementXMLSubmission_2015-06-02_01:55:01.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000605
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMarc Vaudel
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiTRAQ4plex-114 reporter+balance reagent O4'-acylated tyrosine: 144.105918; 2-pyrrolidone-5-carboxylic acid (Glu): -18.010565; acetylated residue: 42.010565; 2-pyrrolidone-5-carboxylic acid (Gln): -17.026549; monohydroxylated residue: 15.994915; iTRAQ4plex-114 reporter+balance reagent N6-acylated lysine: 144.105918; iTRAQ4plex-114 reporter+balance reagent acylated N-terminal: 144.105918; L-cysteine methyl disulfide: 45.987721
InstrumentLTQ Orbitrap; instrument model: LTQ-Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-11-27 02:56:48ID requested
12014-09-16 06:44:04announced
22015-06-02 01:55:03announcedUpdated publication reference for PubMed record(s): 25333471.
Publication List
Helgeland E, Breivik LE, Vaudel M, Svendsen Ø S, Garberg H, Nordrehaug JE, Berven FS, Jonassen AK, Exploring the human plasma proteome for humoral mediators of remote ischemic preconditioning--a word of caution. PLoS One, 9(10):e109279(2014) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: human, plasma, remote ischemic preconditioning
Contact List
Marc Vaudel
contact affiliationPROBE Dept of Biomedicine
contact emailmarc.vaudel@biomed.uib.no
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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