Updated publication reference for PubMed record(s): 25333471. Six healthy male adults underwent RIPC, consisting of 3x5 min cycles of upper arm ischemia (inflating a blood pressure cuff to >200 mmHg) alternating with 3x5 min reperfusion (bp cuff deflated). Blood samples were collected at baseline and at 1 and 4 min into each reperfusion period. Plasma was isolated and the six reperfusion samples were pooled to one RIPC sample. Both baseline and RIPC samples were depleted, concentrated, trypsin digested, iTRAQ labeled and analyzed by LC and Orbitrap-MS where each RIPC sample was compared to the same individual's baseline sample.