PXD000578 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry-based quantitative proteomics of whole cell lysate from breast cancer cell line MCF7 spiked with 57 protein standards |
Description | Here we present quantitative proteomics data used in the evaluation of quantitative accuracy. A human cell line, MCF7 was split into 9 aliquotes that were spiked with a dilution series of 57 protein standards of known amounts spanning 5 orders of magnitude. The protein extracts were trypsinized, and the peptides were analysed by LC-MS using either a label-free or a label-based (TMT 6-plex and iTRAQ 8-plex) quantification approach. The iTRAQ- and TMT-labelled samples were co-analysed and separated by HiRIEF (high resolution isoelectric focusing) prior LC-MS. |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-25 |
AnnouncementXML | Submission_2014-07-25_01:46:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | AnnSofi Sandberg |
SpeciesList | scientific name: Homo Sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; iTRAQ8plex:13C(6)15N(2); TMT6plex; iTRAQ8plex |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-11-13 01:22:24 | ID requested | |
1 | 2013-12-03 07:20:18 | announced | |
1 | 2013-12-03 07:21:14 | announced | |
⏵ 2 | 2014-07-25 01:46:57 | announced | Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: iTRAQ,TMT,quantification,MCF7,LC-MS,precursor mixing,isoelectric focusing |
Contact List
AnnSofi Sandberg |
contact affiliation | Karolinska Institutet |
contact email | annsofi.sandberg@ki.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000578
- Label: PRIDE project
- Name: Mass spectrometry-based quantitative proteomics of whole cell lysate from breast cancer cell line MCF7 spiked with 57 protein standards