PXD000549 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics of the Neisseria gonorrhoeae cell envelope and membrane vesicles |
Description | Neisseria gonorrhoeae (GC) is a human-specific pathogen, and the agent of a sexually transmitted disease, gonorrhea. There is a critical need for new approaches to study and treat GC infections because of the growing threat of multidrug-resistant isolates and the lack of a vaccine. Despite the implied role of the GC cell envelope and membrane vesicles in colonization and infection of human tissues and cell lines, comprehensive studies have not been undertaken to elucidate their constituents. Accordingly, in pursuit of novel molecular therapeutic targets, we have applied isobaric tagging for absolute quantification coupled with liquid chromatography and mass spectrometry for proteome quantitative analyses. Mining the proteome of cell envelopes and native membrane vesicles revealed 533 and 168 common proteins, respectively, in analyzed GC strains FA1090, F62, MS11, and 1291. |
HostingRepository | PRIDE |
AnnounceDate | 2014-12-19 |
AnnouncementXML | Submission_2014-12-19_08:37:45.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Aleksandra Sikora |
SpeciesList | scientific name: Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090); NCBI TaxID: 242231; |
ModificationList | iTRAQ4plex-116 reporter+balance reagent acylated residue; L-cysteine methyl disulfide |
Instrument | 4800 Proteomics Analyzer |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-10-29 03:51:03 | ID requested | |
⏵ 1 | 2014-12-19 08:37:46 | announced | |
Publication List
Zielke RA, Wierzbicki IH, Weber JV, Gafken PR, Sikora AE, Quantitative proteomics of the Neisseria gonorrhoeae cell envelope and membrane vesicles for the discovery of potential therapeutic targets. Mol Cell Proteomics, 13(5):1299-317(2014) [pubmed] |
Keyword List
submitter keyword: iTRAQ, Neisseria gonorrhoeae, cell envelope, native membrane vesicles, drug and vaccine targets |
Contact List
Aleksandra E. Sikora |
contact affiliation | Oregon State University |
contact email | aleksandra.sikora@oregonstate.edu |
lab head | |
Aleksandra Sikora |
contact affiliation | College of Pharmacy |
contact email | aleksandra.sikora@oregonstate.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000549
- Label: PRIDE project
- Name: Quantitative proteomics of the Neisseria gonorrhoeae cell envelope and membrane vesicles