PXD000549

PXD000549 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of the Neisseria gonorrhoeae cell envelope and membrane vesicles
Description	Neisseria gonorrhoeae (GC) is a human-specific pathogen, and the agent of a sexually transmitted disease, gonorrhea. There is a critical need for new approaches to study and treat GC infections because of the growing threat of multidrug-resistant isolates and the lack of a vaccine. Despite the implied role of the GC cell envelope and membrane vesicles in colonization and infection of human tissues and cell lines, comprehensive studies have not been undertaken to elucidate their constituents. Accordingly, in pursuit of novel molecular therapeutic targets, we have applied isobaric tagging for absolute quantification coupled with liquid chromatography and mass spectrometry for proteome quantitative analyses. Mining the proteome of cell envelopes and native membrane vesicles revealed 533 and 168 common proteins, respectively, in analyzed GC strains FA1090, F62, MS11, and 1291.
HostingRepository	PRIDE
AnnounceDate	2014-12-19
AnnouncementXML	Submission_2014-12-19_08:37:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Sikora
SpeciesList	scientific name: Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090); NCBI TaxID: 242231;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; L-cysteine methyl disulfide
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-29 03:51:03	ID requested
⏵ 1	2014-12-19 08:37:46	announced

Zielke RA, Wierzbicki IH, Weber JV, Gafken PR, Sikora AE, Quantitative proteomics of the Neisseria gonorrhoeae cell envelope and membrane vesicles for the discovery of potential therapeutic targets. Mol Cell Proteomics, 13(5):1299-317(2014) [pubmed]

submitter keyword: iTRAQ, Neisseria gonorrhoeae, cell envelope, native membrane vesicles, drug and vaccine targets

Aleksandra E. Sikora
contact affiliation	Oregon State University
contact email	aleksandra.sikora@oregonstate.edu
lab head
Aleksandra Sikora
contact affiliation	College of Pharmacy
contact email	aleksandra.sikora@oregonstate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/12/PXD000549

PRIDE project URI

• PRIDE
  1. PXD000549
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of the Neisseria gonorrhoeae cell envelope and membrane vesicles