PXD000513 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Photo-cross-linking mass spectrometry for single amino acid assignment of RNA-binding sites in RNA-binding proteins |
Description | Mass spectrometry data of peptide–RNA cross-links derived from complex biological systems. Yeast (pre-)mRNPs were isolated by TAP tag purification of Cbp20; human protein–RNA complexes were assembled on an aptamer tagged pre-mRNA. Isolated complexes were UV irradiated. For each of the aforementioned experiments, a non-irradiated control was prepared. Yeast RBPs cross-linked in vivo after labeling with 4-thio-uridine (4SU) were isolated with oligo d(T). All samples were enriched for cross-linked heteroconjugates prior to LC-MS analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2014-08-07 |
AnnouncementXML | Submission_2014-08-07_06:52:34.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Timo Sachsenberg |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-10-11 07:31:57 | ID requested | |
1 | 2014-08-07 01:12:09 | announced | |
⏵ 2 | 2014-08-07 06:52:34 | announced | Updated project metadata. |
3 | 2014-10-23 02:44:29 | announced | Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: human, RNP, cross-linking, mass spectrometry |
Contact List
Oliver Kohlbacher |
contact affiliation | University of Tübingen |
contact email | oliver.kohlbacher@uni-tuebingen.de |
lab head | |
Timo Sachsenberg |
contact affiliation | University of Tübingen |
contact email | sachsenb@informatik.uni-tuebingen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD000513 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000513
- Label: PRIDE project
- Name: Photo-cross-linking mass spectrometry for single amino acid assignment of RNA-binding sites in RNA-binding proteins